Structure and phycobiliprotein composition of phycobilisomes from Griffithsia pacifica (Rhodophyceae)

Journal Article · Mon Sep 01 04:00:00 EDT 1980 · J. Phycol.; (United States)

DOI:https://doi.org/10.1111/j.1529-8817.1980.tb03051.x· OSTI ID:7097784

Gantt, E; Lipschultz, C A

Phycobilisomes in Griffithsia pacifica are closely spaced on the thylakoid membrane. By negative staining, attached and isolated phycobilisomes have been shown to have a block shaped appearance. They are 63 nm long, 38 nm high, and 38 nm wide, making them the largest thus far reported. Isolated phycobilisomes, shown to be functionally intact by their 675 nm fluorescence emission (excitation 545 nm) were stable for more than a day. Phycobiliproteins from dissociated phycobilisomes, separated on sucrose gradients and by polyacrylamide, electrophoresis, yielded large (R-) and small (r-) molecular weight species of phycoerythrin (ca. 4:1 respectively) constituting 89% of the phycobiliprotein content, with R-phycocyanin 8%, and allophycocyanin 3% accounting for the rest. Phycobilisomes of Griffithsia pacifica and Porphyridium purpureum (Bory) Drew and Ross (P. cruentum) are structurally very similar with phycoerythrin being on the outside and surrounding a core of R-phycocyanin and allophycocyanin.

• Research Organization: Smithsonian Institution, Rockville, MD
• OSTI ID: 7097784
• Journal Information: J. Phycol.; (United States), Journal Name: J. Phycol.; (United States) Vol. 16:3; ISSN JPYLA
• Country of Publication: United States
• Language: English

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59 BASIC BIOLOGICAL SCIENCES
ALGAE
CELL CONSTITUENTS
CELL MEMBRANES
CHEMICAL ANALYSIS
CHEMICAL COMPOSITION
CONFIGURATION
ELECTROPHORESIS
FLUORESCENCE
LUMINESCENCE
MEMBRANES
MOLECULAR STRUCTURE
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