Probing phycobilisome structure by immuno-electron microscopy
Journal Article
·
· J. Phycol.; (United States)
By immuno-electron microscopy it was shown that phycoerythrin is located on the outer surface of the phycobilisome and allophycocyanin is on the inside near the photosynthetic membrane in the red alga Porphyridium purpureum (Bory) Drew and Ross (P. cruentum). These findings are consistent with the idea that the phycobilisome functions as a light harvesting antennae and energy sink, which directs the energy to chlorophyll in the photosynthetic membrane. A technique was devised in which unfixed phycobilisomes, attached to thylakoid vesicles, were separately reacted with three monospecific antisera (to B-phycoerythrin, R-phycocyanin and allophycocyanin) and the reaction products were secondarily marked by reaction with ferritin-conjugated goat-antirabbit gamma globulin fraction. This was subsequently followed by glutaraldehyde fixation and staining with phosphotungstic acid. The entire procedure was carried out on an electron microscope grid. The results confirm the previously proposed phycobilisome structural model.
- Research Organization:
- Smithsonian Institution, Rockville, MD
- OSTI ID:
- 6753466
- Journal Information:
- J. Phycol.; (United States), Journal Name: J. Phycol.; (United States) Vol. 13; ISSN JPYLA
- Country of Publication:
- United States
- Language:
- English
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Primary light-harvesting system: phycobilisomes and associated membranes. Progress report, January 1, 1981-December 31, 1981
Journal Article
·
Mon Sep 01 00:00:00 EDT 1980
· J. Phycol.; (United States)
·
OSTI ID:7097784
Phycobilisome structure of porphyridium cruentum: polypeptide composition
Journal Article
·
Wed Dec 31 23:00:00 EST 1980
· Plant Physiol.; (United States)
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OSTI ID:7192447
Primary light-harvesting system: phycobilisomes and associated membranes. Progress report, January 1, 1981-December 31, 1981
Technical Report
·
Wed Dec 31 23:00:00 EST 1980
·
OSTI ID:6113619
Related Subjects
550300* -- Cytology
59 BASIC BIOLOGICAL SCIENCES
ALGAE
BIOLOGICAL LOCALIZATION
BIOMASS
CARBOXYLIC ACIDS
CELL CONSTITUENTS
CELL MEMBRANES
CHEMICAL REACTIONS
CHLOROPHYLL
COMPLEXES
CYTOLOGICAL TECHNIQUES
ELECTRON MICROSCOPY
ENERGY SOURCES
FERRITIN
GLOBULINS
GLOBULINS-GAMMA
HETEROCYCLIC ACIDS
HETEROCYCLIC COMPOUNDS
IMMUNE REACTIONS
IRON COMPLEXES
MEMBRANES
MICROSCOPY
MORPHOLOGY
ORGANIC ACIDS
ORGANIC COMPOUNDS
ORGANIC NITROGEN COMPOUNDS
PHOTOCHEMICAL REACTIONS
PHOTOSYNTHESIS
PHYCOCYANIN
PHYTOCHROMES
PIGMENTS
PLANT CELLS
PLANTS
PORPHYRINS
PROTEINS
RENEWABLE ENERGY SOURCES
SYNTHESIS
TRANSITION ELEMENT COMPLEXES
59 BASIC BIOLOGICAL SCIENCES
ALGAE
BIOLOGICAL LOCALIZATION
BIOMASS
CARBOXYLIC ACIDS
CELL CONSTITUENTS
CELL MEMBRANES
CHEMICAL REACTIONS
CHLOROPHYLL
COMPLEXES
CYTOLOGICAL TECHNIQUES
ELECTRON MICROSCOPY
ENERGY SOURCES
FERRITIN
GLOBULINS
GLOBULINS-GAMMA
HETEROCYCLIC ACIDS
HETEROCYCLIC COMPOUNDS
IMMUNE REACTIONS
IRON COMPLEXES
MEMBRANES
MICROSCOPY
MORPHOLOGY
ORGANIC ACIDS
ORGANIC COMPOUNDS
ORGANIC NITROGEN COMPOUNDS
PHOTOCHEMICAL REACTIONS
PHOTOSYNTHESIS
PHYCOCYANIN
PHYTOCHROMES
PIGMENTS
PLANT CELLS
PLANTS
PORPHYRINS
PROTEINS
RENEWABLE ENERGY SOURCES
SYNTHESIS
TRANSITION ELEMENT COMPLEXES