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Multiple bidirectional alterations of phenotype and changes in proliferative potential during the in vitro and in vivo passage of clonal mast cell populations derived from mouse peritoneal mast cells

Journal Article · · Blood; (United States)
OSTI ID:7095692
; ; ; ; ; ; ;

Mouse peritoneal mast cells (PMC) express a connective tissue-type mast cell (CTMC) phenotype, including reactivity with the heparin-binding fluorescent dye berberine sulfate and incorporation of (35S) sulfate predominantly into heparin proteoglycans. When PMC purified to greater than 99% purity were cultured in methylcellulose with IL-3 and IL-4, approximately 25% of the PMC formed colonies, all of which contained both berberine sulfate-positive and berberine sulfate-negative mast cells. When these mast cells were transferred to suspension culture, they generated populations that were 100% berberine sulfate-negative, a characteristic similar to that of mucosal mast cells (MMC), and that synthesized predominantly chondroitin sulfate (35S) proteoglycans. When ''MMC-like'' cultured mast cells derived from WBB6F1-+/+ PMC were injected into the peritoneal cavities of mast cell-deficient WBB6F1-W/Wv mice, the adoptively transferred mast cell population became 100% berberine sulfate-positive. In methylcellulose culture, these ''second generation PMC'' formed clonal colonies containing both berberine sulfate-positive and berberine sulfate-negative cells, but exhibited significantly less proliferative ability than did normal +/+ PMC. Thus, clonal mast cell populations initially derived from single PMC exhibited multiple and bidirectional alterations between CTMC-like and MMC-like phenotypes. However, this process was associated with a progressive diminution of the mast cells' proliferative ability.

Research Organization:
Osaka Univ. Medical School (Japan)
OSTI ID:
7095692
Journal Information:
Blood; (United States), Journal Name: Blood; (United States) Vol. 72:3; ISSN BLOOA
Country of Publication:
United States
Language:
English

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Related Subjects

550201* -- Biochemistry-- Tracer Techniques
59 BASIC BIOLOGICAL SCIENCES
AMINES
ANIMAL CELLS
ANIMALS
AZOLES
BETA DECAY RADIOISOTOPES
BETA-MINUS DECAY RADIOISOTOPES
BIOSYNTHESIS
CARBOHYDRATES
CELL CULTURES
CELL PROLIFERATION
CLONE CELLS
COLONY FORMATION
CONNECTIVE TISSUE CELLS
CULTURE MEDIA
DAYS LIVING RADIOISOTOPES
EVEN-ODD NUCLEI
FLUORESCENCE
GLUCOPROTEINS
GLYCOPROTEINS
GROWTH FACTORS
HETEROCYCLIC COMPOUNDS
HISTAMINE
IMIDAZOLES
IN VITRO
IN VIVO
ISOTOPE APPLICATIONS
ISOTOPES
LIGHT NUCLEI
LUMINESCENCE
LYMPHOKINES
MAMMALS
MAST CELLS
MEMBRANES
MICE
MITOGENS
NUCLEI
ORGANIC COMPOUNDS
ORGANIC NITROGEN COMPOUNDS
OXYGEN COMPOUNDS
PERITONEUM
PHENOTYPE
PROTEINS
RADIOISOTOPES
RODENTS
SACCHARIDES
SEROUS MEMBRANES
SOMATIC CELLS
SULFATES
SULFUR 35
SULFUR COMPOUNDS
SULFUR ISOTOPES
SYNTHESIS
TRACER TECHNIQUES
VERTEBRATES