Quantification of phenylethylamine and p-tyramine in rat tissues using a new radioenzymatic assay
Phenylethylamine (PEA) and p-tyramine (p-tym) are biologically active aralkylamines that are found in a number of mammalian tissues, including brain and plasma. The investigation of the biological role of these substances has been hampered by the lack of accessible assay methodology. They have developed a new radioenzymatic assay using barley root tyramine N-methyltransferase and tritiated S-adenosylmethionine. The products formed by the reaction are isolated by TLC. The assay sensitivity was 2.1 and 1.0 pg/tube for PEA and p-tym, respectively. The concentration of PEA and p-tym was determined simultaneously in tissues from Sprague-Dawley rats (280 gm). Plasma PEA and p-tym were 478 +/- 66 and 309 +/- 69 pg/ml, respectively. They conclude that this new procedure is applicable to all tissues examined in that all tissues contain both PEA and p-tym and that these amines are heterogeneously distributed in rat tissues.
- Research Organization:
- Indiana Univ. School of Medicine, Indianapolis
- OSTI ID:
- 7092988
- Report Number(s):
- CONF-8604222-; TRN: 87-008607
- Journal Information:
- Fed. Proc., Fed. Am. Soc. Exp. Biol.; (United States), Vol. 45:4; Conference: 70. annual meeting of the Federation of American Society for Experimental Biology, St. Louis, MO, USA, 13 Apr 1986
- Country of Publication:
- United States
- Language:
- English
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Related Subjects
AMINES
RADIOENZYMATIC ASSAY
TISSUE DISTRIBUTION
TYRAMINE
BARLEY
METHIONINE
RATS
ROOTS
THIN-LAYER CHROMATOGRAPHY
TRACER TECHNIQUES
TRANSFERASES
TRITIUM COMPOUNDS
AMINO ACIDS
ANIMALS
AROMATICS
AUTONOMIC NERVOUS SYSTEM AGENTS
CARBOXYLIC ACIDS
CEREALS
CHROMATOGRAPHY
DISTRIBUTION
DRUGS
ENZYMES
GRASS
HYDROXY COMPOUNDS
ISOTOPE APPLICATIONS
LABELLED COMPOUNDS
LIPOTROPIC FACTORS
MAMMALS
ORGANIC ACIDS
ORGANIC COMPOUNDS
ORGANIC SULFUR COMPOUNDS
PHENOLS
PLANTS
RODENTS
SEPARATION PROCESSES
SYMPATHOMIMETICS
VERTEBRATES
550201* - Biochemistry- Tracer Techniques