Tyramine production by the isolated perfused rat kidney: modulation by perfusate tyrosine concentration

Conference · Sat Mar 01 04:00:00 EST 1986 · Fed. Proc., Fed. Am. Soc. Exp. Biol.; (United States)

OSTI ID:5497255

Brier, M E; Bowsher, R R; Mayer, P R; Henry, D P

The urinary excretion rate of p-tyramine (p-tym) is greater than that of other neurohumoral aralkylamines. The source and significance of urinary p-tym are not characterized. Isolated rat kidneys (n = 12) were perfused in vitro in a 70 ml recirculating reservoir for 60 min. Perfusate (tyr) was varied. Tym was quantified using a new radioenzymatic assay with a sensitivity of 0.8 pg. p-Tym synthesis, urinary excretion, and concentration in perfusate increased with perfusate tyr concentration. Even though > 80% of synthesized p-tym was excreted, perfusate concentration of p-tym reach > 1000x the plasma concentration of tym (0.5 ng/ml) found in vivo. The authors conclude that decarboxylation of tyr by the kidney is the major source of urinary p-tym, renal synthesis of tym could contribute significantly to plasma tym, and tyr delivery to kidney modulates tym synthesis.

• Research Organization: Indiana Univ. School of Medicine, Indianapolis
• OSTI ID: 5497255
• Report Number(s): CONF-8604222-
• Conference Information: Journal Name: Fed. Proc., Fed. Am. Soc. Exp. Biol.; (United States) Journal Volume: 45:3
• Country of Publication: United States
• Language: English

Related Subjects

550201* -- Biochemistry-- Tracer Techniques
59 BASIC BIOLOGICAL SCIENCES
AMINES
ANIMALS
AROMATICS
AUTONOMIC NERVOUS SYSTEM AGENTS
BIOLOGICAL MATERIALS
BIOLOGICAL WASTES
BIOSYNTHESIS
BODY
BODY FLUIDS
CLEARANCE
DRUGS
EXCRETION
HYDROXY COMPOUNDS
KIDNEYS
MAMMALS
MATERIALS
ORGANIC COMPOUNDS
ORGANS
PERFUSED ORGANS
PHENOLS
RADIOENZYMATIC ASSAY
RATS
RODENTS
SYMPATHOMIMETICS
SYNTHESIS
TYRAMINE
URINE
VERTEBRATES
WASTES