Conversion of p-tyrosine to p-tyramine in the isolated perfused rat kidney: Modulation by perfusate concentrations of p-tyrosine

Journal Article · Tue Jan 01 04:00:00 EST 1991 · Life Sciences; (USA)

DOI:https://doi.org/10.1016/0024-3205(91)90037-C· OSTI ID:5763145

Brier, M E ^[1]; Bowsher, R R; Henry, D P ^[2]; Mayer, P R ^[3]

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1. Univ. of Louisville, KY (USA) Purdue Univ., West Lafayette, IN (USA)
2. Eli Lilly and Co., Indianapolis, IN (USA)
3. Purdue Univ., West Lafayette, IN (USA)

The authors used the isolated perfused rat kidney to evaluate the role of renal decarboxylation of p-tyrosine as the source of urinary p-tyramine. Kidneys were perfused with concentrations of p-tyrosine ranging from 0.02 mM to 2.0 mM. p-Tyramine was measured by a sensitive and specific radioenzymatic assay. An increase in the perfusate concentration of p-tyrosine resulted in a significant increase in p-tyramine production that was blocked by the addition of NSD-1015, an inhibitor of aromatic-1-amino decarboxylase (AADC). They conclude p-tyrosine is the precursor for the renal production of p-tyramine, renal AADC catalyzes the formation of urinary p-tyramine, synthesized p-tyramine is predominantly excreted in the urine, and p-tyramine synthesis is modulated by the arterial delivery of p-tyrosine to the kidney.

• OSTI ID: 5763145
• Journal Information: Life Sciences; (USA), Journal Name: Life Sciences; (USA) Vol. 48:9; ISSN 0024-3205; ISSN LIFSA
• Country of Publication: United States
• Language: English

Related Subjects

550201* -- Biochemistry-- Tracer Techniques
59 BASIC BIOLOGICAL SCIENCES
AMINES
AMINO ACIDS
ANIMALS
AROMATICS
AUTONOMIC NERVOUS SYSTEM AGENTS
BIOSYNTHESIS
BODY
CARBOXYLIC ACIDS
CHEMICAL REACTIONS
DECARBOXYLATION
DRUGS
ENZYME INHIBITORS
HYDROXY ACIDS
HYDROXY COMPOUNDS
ISOTOPE APPLICATIONS
KIDNEYS
MAMMALS
ORGANIC ACIDS
ORGANIC COMPOUNDS
ORGANS
PERFUSED ORGANS
PHENOLS
RADIOENZYMATIC ASSAY
RATS
RODENTS
SYMPATHOMIMETICS
SYNTHESIS
TRACER TECHNIQUES
TYRAMINE
TYROSINE
VERTEBRATES