/sup 22/Na+ and /sup 86/Rb+ transport in vascular smooth muscle of SHR, Wistar Kyoto, and Wistar rats
To gain further insight into differences in cellular Na+ and K+ regulation between the spontaneously hypertensive rat (SHR), Wistar Kyoto (WKY), and American Wistar (W) rats, 22Na+ and 86Rb+ washouts were performed under steady-state conditions in cultured vascular smooth muscle cells from the three rat strains. SHR vascular smooth muscle cells showed significantly higher bumetanide sensitive 86Rb+ washout rate constant (x 10(-4)/min; mean +/- SEM) than WKY cells (-38.6 +/- 2.84 and -23.8 +/- 3.58, respectively; p less than 0.005). SHR vascular smooth muscle cells also exhibited significantly higher values than WKY cells in the total 22Na+ washout rate constant (x 10(-2)/min) (-61.0 +/- 1.57 vs. -53.8 +/- 1.24; p less than 0.005). The amiloride sensitive component of the 22Na+ washout rate constant accounted for these differences (-18.6 +/- 1.04 for SHR and -12.1 +/- 2.00 for WKY; p less than 0.05). There were no apparent differences in cellular Na+ concentrations between WKY and SHR cells. In general, the 86Rb+ and 22Na+ washout parameters of W rat cells were quite similar to those of cells from SHR. We conclude that the bumetanide-sensitive 86Rb+ washout (the Na+ K+-cotransport), the overall, and the amiloride-sensitive 22Na+ washout (the latter primarily represents the Na+/H+ antiport) are higher in SHR than WKY rat vascular smooth muscle cells. These findings indicate innate differences in cellular Na+ and K+ transport in vascular smooth muscle cells of the SHR and WKY rat. The mechanisms responsible for these differences are yet to be determined.
- Research Organization:
- New Jersey Medical School, Newark (USA)
- OSTI ID:
- 7092864
- Journal Information:
- J. Cardiovasc. Pharmacol.; (United States), Vol. 11:6
- Country of Publication:
- United States
- Language:
- English
Similar Records
Spontaneously hypertensive rat vascular smooth muscle cells in culture exhibit increased growth and Na+/H+ exchange
Reduced Na-K-Cl cotransport in vascular smooth muscle cells from spontaneously hypertensive rats
Related Subjects
HYPERTENSION
PATHOGENESIS
RUBIDIUM COMPOUNDS
MEMBRANE TRANSPORT
SODIUM COMPOUNDS
CATIONS
CELL CULTURES
GENETIC VARIABILITY
IN VITRO
MUSCLES
OUABAIN
RATS
RUBIDIUM 86
SODIUM 22
STEADY-STATE CONDITIONS
TRACER TECHNIQUES
ALKALI METAL COMPOUNDS
ALKALI METAL ISOTOPES
ANIMALS
BETA DECAY RADIOISOTOPES
BETA-MINUS DECAY RADIOISOTOPES
BETA-PLUS DECAY RADIOISOTOPES
BIOLOGICAL VARIABILITY
CARBOHYDRATES
CARDIAC GLYCOSIDES
CARDIOTONICS
CARDIOVASCULAR AGENTS
CARDIOVASCULAR DISEASES
CHARGED PARTICLES
DAYS LIVING RADIOISOTOPES
DISEASES
DRUGS
GLYCOSIDES
INTERMEDIATE MASS NUCLEI
IONS
ISOMERIC TRANSITION ISOTOPES
ISOTOPE APPLICATIONS
ISOTOPES
LIGHT NUCLEI
MAMMALS
MINUTES LIVING RADIOISOTOPES
NUCLEI
ODD-ODD NUCLEI
ORGANIC COMPOUNDS
RADIOISOTOPES
RODENTS
RUBIDIUM ISOTOPES
SODIUM ISOTOPES
STROPHANTHINS
SYMPTOMS
VASCULAR DISEASES
VERTEBRATES
YEARS LIVING RADIOISOTOPES
550901* - Pathology- Tracer Techniques