Bumetanide-sensitive sodium-22 transport in vascular smooth muscle cell of the spontaneously hypertensive rat

Journal Article · Thu May 01 04:00:00 EDT 1986 · Hypertension (Dallas); (United States)

DOI:https://doi.org/10.1161/01.HYP.8.5.379· OSTI ID:5618336

Tokushige, A; Kino, M; Tamura, H; Hopp, L; Searle, B M; Aviv, A

The effect of bumetanide, a known probe of Na+, K+ cotransport, on /sup 22/Na+ uptake and washout was examined in serially passed cultured vascular smooth muscle cells of spontaneously hypertensive rats (SHR), Wistar-Kyoto rats (WKY), and Wistar rats. In Ca2+-deficient medium, the drug exerted the greatest effect on /sup 22/Na+ washout in vascular smooth muscle cells from SHR and the least effect on cells from WKY. The respective mean values for the apparent bumetanide-sensitive /sup 22/Na+ washout rate constants (Ke; X 10(-2)/min) were 7.2, 4.3, and 1.7 for cells from SHR, WKY, and Wistar rats. In both 1 mM Ca2+ and Ca2+-deficient medium, in the presence of 1 mM ouabain, vascular smooth muscle cells from SHR had the highest plateau phase of /sup 22/Na+ uptake among the three cell preparations. All cells exhibited higher /sup 22/Na+ uptake in Ca2+-deficient medium than in 1 mM Ca2+ medium. Under this condition, bumetanide caused an additional rise in steady state /sup 22/Na+ uptake that was most pronounced in cells from SHR (21.3% versus 16.6% for Wistar rats and 4.8% for WKY). This finding indicates that a quantitatively greater inhibition of washout than of the uptake component of the bumetanide-sensitive /sup 22/Na+ transport occurs in Ca2+-deficient medium. It is concluded that, in Ca2+-deficient medium, the bumetanide-sensitive /sup 22/Na+ washout is higher in vascular smooth muscle cells of SHR than in those of normotensive controls and that this phenomenon reflects a higher Na+ turnover in vascular smooth muscle cell in the hypertensive rat strain.

• Research Organization: New Jersey Medical School, Newark
• OSTI ID: 5618336
• Journal Information: Hypertension (Dallas); (United States), Journal Name: Hypertension (Dallas); (United States) Vol. 5; ISSN HPRTD
• Country of Publication: United States
• Language: English

Related Subjects

550901* -- Pathology-- Tracer Techniques
59 BASIC BIOLOGICAL SCIENCES
ALKALI METAL ISOTOPES
ALKALI METALS
ALKALINE EARTH METALS
ANIMALS
BETA DECAY RADIOISOTOPES
BETA-PLUS DECAY RADIOISOTOPES
BIOCHEMICAL REACTION KINETICS
BIOLOGICAL MATERIALS
BLOOD
BLOOD CELLS
BODY FLUIDS
CALCIUM
CARBOHYDRATES
CARDIAC GLYCOSIDES
CARDIOTONICS
CARDIOVASCULAR AGENTS
CARDIOVASCULAR DISEASES
CELL CULTURES
DISEASES
DRUGS
ELEMENTS
ERYTHROCYTES
GLYCOSIDES
HYPERTENSION
ISOTOPE APPLICATIONS
ISOTOPES
KINETICS
LIGHT NUCLEI
MAMMALS
MATERIALS
MEMBRANE TRANSPORT
METALS
MUSCLES
NUCLEI
ODD-ODD NUCLEI
ORGANIC COMPOUNDS
OUABAIN
PATHOGENESIS
RADIOISOTOPES
RATS
REACTION KINETICS
RODENTS
SODIUM
SODIUM 22
SODIUM ISOTOPES
STROPHANTHINS
SYMPTOMS
TRACER TECHNIQUES
VASCULAR DISEASES
VERTEBRATES
YEARS LIVING RADIOISOTOPES