Comparison of the degree of homology of DNA and quantity of repeated sequences in an intact plant and cell structure

Journal Article · Mon Sep 01 00:00:00 EDT 1986 · Dokl. Biol. Sci. (Engl. Transl.); (United States)

OSTI ID:7091868

Solov'yan, V T; Kunaleh, V A; Shumnyl, V K; Vershinin, A V

This paper attempts to assess the quantity of repeated sequences and degree of homology of DNA in the intact plant and two lines of callus tissue of Rauwolfia serpentina Benth maintained for 20 years, which differ among themselves in the level of biosynthesis of the pharmacologically valuable alkaloid ajmaline. The tritium-labeled repeats of plants and calli were used in direct and reverse hybridization on nitrocellulose filters. Hybridization of H 3-labeled repeats with phage 17 DNA was used as control. The radioactivity of filters after washing was measured in a liquid scintillation counter.

• Research Organization: Institute of Molecular Biology, Acad. of Sci., Kiev
• OSTI ID: 7091868
• Journal Information: Dokl. Biol. Sci. (Engl. Transl.); (United States), Vol. 287:1-6
• Country of Publication: United States
• Language: English

Related Subjects

59 BASIC BIOLOGICAL SCIENCES
DNA
BIOSYNTHESIS
TRACER TECHNIQUES
PLANT CELLS
DNA SEQUENCING
ALKALOIDS
CELL CULTURES
GENOME MUTATIONS
HYBRIDIZATION
NITROCELLULOSE
NUCLEOSIDES
PHARMACOLOGY
PLANT TISSUES
PLOIDY
SCINTILLATION COUNTING
TRITIUM COMPOUNDS
BODY
CARBOHYDRATES
CELLULOSE ESTERS
CHEMICAL EXPLOSIVES
COUNTING TECHNIQUES
ESTERS
EXPLOSIVES
ISOTOPE APPLICATIONS
LABELLED COMPOUNDS
MUTATIONS
NITRIC ACID ESTERS
NUCLEIC ACIDS
NUCLEOTIDES
ORGANIC COMPOUNDS
POLYSACCHARIDES
RIBOSIDES
SACCHARIDES
STRUCTURAL CHEMICAL ANALYSIS
SYNTHESIS
TISSUES
550401* - Genetics- Tracer Techniques