Partial replication of UV-irradiated T4 bacteriophage DNA results in amplification of specific genetic areas

Journal Article · Sun Nov 01 04:00:00 EST 1981 · J. Virol.; (United States)

OSTI ID:5363934

Ling, S K; Vogelbacker, H H; Restifo, L L; Mattson, T; Kozinski, A W

Upon infection of Escherichia coli with bromodeoxyuridine-labeled t4 phage that had received 10 lethal hits of UV irradiation, a sizable amount of phage DNA was synthesized (approximately 36 phage equivalent units of DNA per infected bacterium), although very little multiplicity reactivation occurs. This progeny DNA was isolated and analyzed. This DNA was biased in its genetic representation, as shown by hybridization to cloned segments of the T4 genome immobilized on nitrocellulose filters. Preferentially amplified areas corresponded to regions containing origins of T4 DNA replication. The size of the progeny DNA increased with time after infection, possibly due to recombination between partial replicas and nonreplicated subunits or due to the gradual overcoming of the UV damage. As the size of the progeny DNA increased, all of the genes were more equally represented, resulting in a decrease in the genetic bias. Amplification of specific genetic areas was also observed upon infection with UV-irradiated, nonbromodeoxyuridine-substituted (light) phage. However, the genetic bias observed in this case was not as great as that observed with bromodeoxyuridine-substituted phage. This is most likely due to the higher efficiency of multiplicity reactivation of the light phage.

• Research Organization: Department of Human Genetics, University of Pennyslvania School of Medicine, Philadelphia
• OSTI ID: 5363934
• Journal Information: J. Virol.; (United States), Journal Name: J. Virol.; (United States) Vol. 40:2; ISSN JOVIA
• Country of Publication: United States
• Language: English

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59 BASIC BIOLOGICAL SCIENCES
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
ANTIMETABOLITES
AZINES
BACTERIA
BACTERIOPHAGES
BIOLOGICAL EFFECTS
BIOLOGICAL RADIATION EFFECTS
BROMOURACILS
BUDR
DNA
DNA REPLICATION
DRUGS
ELECTROMAGNETIC RADIATION
ESCHERICHIA COLI
GENES
HETEROCYCLIC COMPOUNDS
HYDROXY COMPOUNDS
MICROORGANISMS
NUCLEIC ACID REPLICATION
NUCLEIC ACIDS
NUCLEOSIDES
NUCLEOTIDES
ORGANIC BROMINE COMPOUNDS
ORGANIC COMPOUNDS
ORGANIC HALOGEN COMPOUNDS
ORGANIC NITROGEN COMPOUNDS
PARASITES
PYRIMIDINES
RADIATION EFFECTS
RADIATIONS
RIBOSIDES
ULTRAVIOLET RADIATION
URACILS
VIRUSES