Brain transfer RNA. II. Analysis of modified nucleosides

Journal Article · Fri Jan 01 04:00:00 EST 1982 · Neurochem. Res.; (United States)

DOI:https://doi.org/10.1007/BF00965070· OSTI ID:7089896

Chaudhary, K D; Carrier-Malhotra, L; Murthy, M R

Transfer RNAs were isolated from rat and calf brains and their nucleosides were analysed by tritium derivative technique. Qualitative changes in the minor nucleoside components were compared on the fluorograms which showed differences in the intensities of spots. Cerebellar and cortical tRNAs were also compared, but revealed no significant quantitative differences in their methylated constituants despite 60% higher methyltransferase activity observed in cerebellum compared to cerebral cortex. An overall similarity was noticed between the relative proportions of the major and minor nucleosides of tRNAs derived from rat or calf brain, expressed as mol%. Brain tRNA was also analysed by two-dimensional polyacrylamide gel electrophoresis which showed qualitative and quantitative changes during postnatal development.

• Research Organization: Departement de Biochimie Faculte de Medecine Universite Laval Quebec, Canada
• OSTI ID: 7089896
• Journal Information: Neurochem. Res.; (United States), Journal Name: Neurochem. Res.; (United States) Vol. 7:1; ISSN NERED
• Country of Publication: United States
• Language: English

Related Subjects

550101 -- Behavioral Biology-- Tracer Techniques
550201* -- Biochemistry-- Tracer Techniques
59 BASIC BIOLOGICAL SCIENCES
ANIMALS
BODY
BRAIN
CATTLE
CENTRAL NERVOUS SYSTEM
CEREBELLUM
CEREBRAL CORTEX
CEREBRUM
COMPARATIVE EVALUATIONS
DOMESTIC ANIMALS
ISOTOPE APPLICATIONS
LABELLED COMPOUNDS
MAMMALS
NERVOUS SYSTEM
NUCLEIC ACIDS
NUCLEOSIDES
NUCLEOTIDES
ORGANIC COMPOUNDS
ORGANS
RATS
RIBOSIDES
RNA
RODENTS
RUMINANTS
STRUCTURAL CHEMICAL ANALYSIS
TRANSFER RNA
TRITIUM COMPOUNDS
VERTEBRATES