Uptake of (/sup 14/C)deoxyglucose into brain of young rats with inherited hydrocephalus

Journal Article · Wed Feb 01 04:00:00 EST 1989 · Exp. Neurol.; (United States)

DOI:https://doi.org/10.1016/0014-4886(89)90082-4· OSTI ID:6268333

Richards, H K; Bucknall, R M; Jones, H C; Pickard, J D

The effect of hydrocephalus on cerebral glucose utilization as reflected by deoxyglucose uptake has been examined in rats with inherited hydrocephalus at 10, 20, and 28 days after birth using a semiquantitative method. Injection of (14C)deoxyglucose intraperitoneally was followed by freezing the brain, sectioning, and quantitative autoradiography of 10 brain regions. Brain (14C) concentration, cortical thickness, and plasma glucose concentrations were measured. Maximal thinning of the cerebral cortex had already occurred by 10 days after birth, although obvious symptoms such as gait disturbance developed after 20 days. In control rats, the cerebral isotope concentration was lower and more homogeneous at 10 days than at 20 or 28 days, which may be a reflection of the use of metabolic substrates other than glucose in younger animals. In order to make comparisons between control and hydrocephalic groups, tissue isotope concentrations were normalized to cerebellar cortex which was not affected by the hydrocephalus at any age. In hydrocephalic rats at 10 and 20 days, the concentration of (14C) was lower in all areas except the inferior colliculi and pons but the reduction was only significant in the sensory-motor cortex at 10 days and in the caudate nuclei at 20 days. By 28 days after birth, all areas except the cerebellum (six cortical regions, inferior colliculi, pons, and caudate) had significantly lower isotope concentrations in the hydrocephalic group. It is concluded that cerebral glucose metabolism is significantly reduced by 28 days after birth in H-Tx rats with congenital hydrocephalus and that less marked reductions occur prior to 28 days.

• Research Organization: Wessex Neurological Centre (England)
• OSTI ID: 6268333
• Journal Information: Exp. Neurol.; (United States), Journal Name: Exp. Neurol.; (United States) Vol. 103:2; ISSN EXNEA
• Country of Publication: United States
• Language: English

Related Subjects

550901* -- Pathology-- Tracer Techniques
59 BASIC BIOLOGICAL SCIENCES
AGE DEPENDENCE
ALDEHYDES
ANIMALS
BODY
BRAIN
CARBOHYDRATES
CARBON 14 COMPOUNDS
CENTRAL NERVOUS SYSTEM
CEREBRAL CORTEX
CEREBRUM
DISEASES
GLUCOSE
HEREDITARY DISEASES
HEXOSES
INJECTION
INTAKE
INTRAPERITONEAL INJECTION
ISOTOPE APPLICATIONS
LABELLED COMPOUNDS
MAMMALS
METABOLISM
MONOSACCHARIDES
NERVOUS SYSTEM
NERVOUS SYSTEM DISEASES
ORGANIC COMPOUNDS
ORGANS
PATHOGENESIS
RATS
RODENTS
SACCHARIDES
TRACER TECHNIQUES
UPTAKE
VERTEBRATES