A modified method for the in vivo labeling of red blood cells with /sup 99m/Tc: concise communication

Journal Article · Thu Apr 01 04:00:00 EST 1982 · J. Nucl. Med.; (United States)

OSTI ID:7089270

Callahan, R J; Froelich, J W; McKusick, K A; Leppo, J; Strauss, H W

The rate of incorporation of /sup 99m/Tc into red blood cells pretinned in vivo was measured by collecting blood samples in stannous DTPA solution, which served as a competing ligand for /sup 99m/Tc. This collection technique permitted a measurement of high-affinity red-cell labeling efficiency at the instant of sampling. At 0.5 min after injection only 62% of technetium is tightly bound to the red cell; this rises to 94.5% at 10 min. Based on the graded labeling of the red cells, the in vivo labeling procedure was modified by isolating pertechnetate and red blood cells tinned in vivo in a syringe during the first 10 min of labeling. The pertechnetate is thus prevented from distributing to extravascular compartments, and 90% of the injected /sup 99m/Tc is firmly bound to red blood cells at the time of injection. In a series of 23 patients, seven were tested with the in vivo method and seven with the modified in vivo method, and nine patients were tested with each method on separate occasions. A decrease in gastric activity and improved image quality were found with the modified method compared with the standard method of in vivo red-cell labeling.

• Research Organization: Massachusetts General Hospital, Boston
• OSTI ID: 7089270
• Journal Information: J. Nucl. Med.; (United States), Journal Name: J. Nucl. Med.; (United States) Vol. 23:4; ISSN JNMEA
• Country of Publication: United States
• Language: English

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59 BASIC BIOLOGICAL SCIENCES
62 RADIOLOGY AND NUCLEAR MEDICINE
BETA DECAY RADIOISOTOPES
BETA-MINUS DECAY RADIOISOTOPES
BIOLOGICAL MATERIALS
BLOOD
BLOOD CELLS
BODY
BODY FLUIDS
COMPARATIVE EVALUATIONS
COUNTING TECHNIQUES
DIAGNOSTIC TECHNIQUES
DIAGNOSTIC USES
DIGESTIVE SYSTEM
ERYTHROCYTES
GASTROINTESTINAL TRACT
HOURS LIVING RADIOISOTOPES
IMAGES
IN VIVO
INTERMEDIATE MASS NUCLEI
ISOMERIC NUCLEI
ISOMERIC TRANSITION ISOTOPES
ISOTOPES
LABELLED COMPOUNDS
LABELLING
MATERIALS
NUCLEI
ODD-EVEN NUCLEI
ORGANS
PATIENTS
RADIOISOTOPE SCANNING
RADIOISOTOPES
SCINTISCANNING
STOMACH
TECHNETIUM 99
TECHNETIUM ISOTOPES
USES
YEARS LIVING RADIOISOTOPES