Development and evaluation of a modified method for the in vivo labeling of human erythrocytes with technetium-99m

Thesis/Dissertation · Thu Jan 01 04:00:00 EST 1981

OSTI ID:6147703

Callahan, R J

Labeling red blood cells with Tc-99m by the standard in vivo method results in significant extravascular radioactivity, which negatively affects the quality of blood pool images. One explanation for this distribution is that technetium-99m is incorporated into red blood cells in vivo over several minutes thus allowing Tc-99m pertechnetate to distribute to the extracellular fluid during the first few minutes after injection. To test this hypothesis, serial blood samples were collected from patients during the in vivo labeling procedure using stannous DTPA as a quenching agent for the labeling reaction. The results indicated that labeling proceeds over several minutes reaching 90% at 4 minutes after technetium-99m injection. In this modified method, in vivo tinned red blood cells are withdrawn into a syringe containing technetium-99m pertechnetate and allowed to incubate for ten minutes before reinjection. At this time, 90% of the Tc-99m is firmly bound to the red blood cells. The results show that the incorporation of technetium-99m into pre-tinned red blood cells in vivo and in vitro is a time-dependent reaction with increased rate and extent of labeling occurring at 37/sup 0/C and with increasing hematocrit. Increased levels of stannous ion and the presence of plasma result in decreased labeling efficiency. The volume of whole blood, activity of Tc-99m and patient condition do not affect labelling efficiency. The interaction between hemoglobin and Tc-99m is rapid and complete.

• Research Organization: Massachusetts Coll. of Pharmacy and Allied Health Sciences, Boston (USA)
• OSTI ID: 6147703
• Country of Publication: United States
• Language: English

Related Subjects

550601* -- Medicine-- Unsealed Radionuclides in Diagnostics
62 RADIOLOGY AND NUCLEAR MEDICINE
AMINO ACIDS
BETA DECAY RADIOISOTOPES
BETA-MINUS DECAY RADIOISOTOPES
BIOLOGICAL MATERIALS
BLOOD
BLOOD CELLS
BODY FLUIDS
CARBOXYLIC ACIDS
CHELATING AGENTS
CHEMICAL PREPARATION
DRUGS
DTPA
ERYTHROCYTES
HOURS LIVING RADIOISOTOPES
IN VIVO
INTERMEDIATE MASS NUCLEI
ISOMERIC NUCLEI
ISOMERIC TRANSITION ISOTOPES
ISOTOPE APPLICATIONS
ISOTOPES
LABELLED COMPOUNDS
LABELLING
MATERIALS
NUCLEI
ODD-EVEN NUCLEI
ORGANIC ACIDS
ORGANIC COMPOUNDS
OXYGEN COMPOUNDS
PERTECHNETATES
RADIOISOTOPES
RADIOPHARMACEUTICALS
RADIOPROTECTIVE SUBSTANCES
SYNTHESIS
TECHNETIUM 99
TECHNETIUM COMPOUNDS
TECHNETIUM ISOTOPES
TRACER TECHNIQUES
TRANSITION ELEMENT COMPOUNDS
YEARS LIVING RADIOISOTOPES