Simultaneous radioassay of serum vitamin B/sub 12/ and folic acid. [/sup 125/I and /sup 57/Co tracer techniques]

Journal Article · Thu Sep 01 04:00:00 EDT 1977 · Clin. Chem.; (United States)

OSTI ID:7086833

Gutcho, S; Mansbach, L

The radioassays of vitamin B/sub 12/ and folic acid can be carried out in a single tube to give the simultaneous assay of both vitamins in 100 ..mu..l of serum. Release of bound vitamins from their endogenous binders and the destruction of these binders are effected by a heating step at pH 9.3. The subsequent binding reactions with hog intrinsic factor and milk binder protein proceed advantageously and simultaneously in the same tube at pH 9.3. A single set of dual reagents replaces two sets of reagents that would normally be used for separate radioassays. Complete separation of bound radioactivities, (/sup 57/Co) cyanocobalamin and /sup 125/I-labeled folate derivative, is obtained in a dual-channel gamma counter with no requirement for any correction for spill-over of counting data. Analytical results are comparable to those found for previously developed individual radioassays. The simultaneous assay has decreased technical time of analysis for these interrelated vitamins by about 50%.

• OSTI ID: 7086833
• Journal Information: Clin. Chem.; (United States), Journal Name: Clin. Chem.; (United States) Vol. 23:9; ISSN CLCHA
• Country of Publication: United States
• Language: English

Related Subjects

550601* -- Medicine-- Unsealed Radionuclides in Diagnostics
62 RADIOLOGY AND NUCLEAR MEDICINE
AMINO ACIDS
BETA DECAY RADIOISOTOPES
BLOOD SERUM
CARBOXYLIC ACIDS
COBALT 57
COBALT ISOTOPES
DAYS LIVING RADIOISOTOPES
ELECTRON CAPTURE RADIOISOTOPES
FOLIC ACID
HETEROCYCLIC COMPOUNDS
HYDROXY COMPOUNDS
INTERMEDIATE MASS NUCLEI
IODINE 125
IODINE ISOTOPES
ISOTOPE APPLICATIONS
ISOTOPES
NUCLEI
ODD-EVEN NUCLEI
ORGANIC ACIDS
ORGANIC COMPOUNDS
ORGANIC NITROGEN COMPOUNDS
PTERIDINES
RADIOASSAY
RADIOISOTOPES
TRACER TECHNIQUES
VITAMIN B GROUP
VITAMIN B-12
VITAMINS