Simultaneous radioassay of folate and vitamin B/sub 12/

Patent · Mon Mar 26 23:00:00 EST 1979

OSTI ID:6296329

Gutcho, S; Mansbach, L

A serum sample is heated at an alkaline pH to release folate and vitamin B/sub 12/ from endogenous binders. A simultaneous radioassay for folate and vitamin B/sub 12/ is effected by contacting the sample with binder for folate, binder for vitamin B/sub 12/, folote labeled with one radioactive isotope and vitamin B/sub 12/ labeled with another radioacitve isotope, followed by separation of bound and free portions, and determination of the radioactivity of at least one of the portions. The amounts of folate and vitamin B/sub 12/ present in the sample may be determined from standard curves.

• Assignee: Becton, Dickinson and Co.
• Patent Number(s): US 4146602
• OSTI ID: 6296329
• Country of Publication: United States
• Language: English

Related Subjects

37 INORGANIC, ORGANIC, PHYSICAL, AND ANALYTICAL CHEMISTRY
400103* -- Radiometric & Radiochemical Procedures-- (-1987)
550201 -- Biochemistry-- Tracer Techniques
59 BASIC BIOLOGICAL SCIENCES
AMINO ACIDS
BETA DECAY RADIOISOTOPES
BLOOD SERUM
CARBOXYLIC ACIDS
CHEMICAL ANALYSIS
COBALT 57
COBALT ISOTOPES
DAYS LIVING RADIOISOTOPES
ELECTRON CAPTURE RADIOISOTOPES
FOLIC ACID
HETEROCYCLIC COMPOUNDS
HYDROXY COMPOUNDS
INTERMEDIATE MASS NUCLEI
IODINE 125
IODINE ISOTOPES
ISOTOPES
LABELLED COMPOUNDS
NUCLEI
ODD-EVEN NUCLEI
ORGANIC ACIDS
ORGANIC COMPOUNDS
ORGANIC NITROGEN COMPOUNDS
PTERIDINES
RADIOASSAY
RADIOISOTOPES
VITAMIN B GROUP
VITAMIN B-12
VITAMINS