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Photoabsorption and photoelectron spectra of enzymes in the vacuum-uv region

Journal Article · · Radiat. Res.; (United States)
DOI:https://doi.org/10.2307/3575724· OSTI ID:7086291
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The photoabsorption spectra and photoelectron yield spectra for the enzymes trypsin, ribonuclease, and lysozyme were measured in the vacuum-uv region between 1050 and 2400 angstroms. The energy distribution curves of photoelectrons were also measured. The samples were in the form of thin films on substrates (lithium fluoride plates for photoabsorption measurements and cover glasses coated with gold for photoelectron measurements). The first ionization potentials for these enzymes were determined from the energy distribution curves of photoelectrons. The integral oscillator strengths for wavelengths ranging from 1050 to 2400 angstroms were derived from the absorption spectra: 168 for trypsin, 100 for ribonuclease, and 126 for lysozyme. It is suggested that a considerable part of the absorption spectra for the wavelength region between the first ionization potential and 1050 angstroms in these enzymes may be attributed to the excitations of highly excited states of peptide linkages.
Research Organization:
Tokyo Inst. of Tech., Japan
OSTI ID:
7086291
Journal Information:
Radiat. Res.; (United States), Journal Name: Radiat. Res.; (United States) Vol. 90:3; ISSN RAREA
Country of Publication:
United States
Language:
English

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Related Subjects

560111* -- Radiation Effects on Biochemicals-- In Vitro-- (-1987)
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
COUNTING TECHNIQUES
ELECTROMAGNETIC RADIATION
ELECTRON SPECTROSCOPY
ENERGY SPECTRA
ENZYMES
ESTERASES
FAR ULTRAVIOLET RADIATION
GLYCOSYL HYDROLASES
HYDROLASES
IONIZATION POTENTIAL
LYSOZYME
O-GLYCOSYL HYDROLASES
ORGANIC COMPOUNDS
PEPTIDE HYDROLASES
PEPTIDES
PHOSPHODIESTERASES
PHOTOELECTRON COUNTING
PHOTOELECTRON SPECTROSCOPY
PROTEINS
RADIATIONS
RNA-ASE
SERINE PROTEINASES
SPECTRA
SPECTROSCOPY
TRYPSIN
ULTRAVIOLET RADIATION