Specific cytotoxicity of 16. cap alpha. -(/sup 125/I)iodoestradiol for estrogen receptor-containing breast cancer cells

Journal Article · Tue Jun 01 00:00:00 EDT 1982 · Endocrinology; (United States)

OSTI ID:7086126

Bronzert, D A ^[1]; Hochberg, R B; Lippman, M E

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1. National Cancer Inst., Bethesda, MD

We attempted to induce specific killing of estrogen receptor-containing breast cancer cells using estradiol coupled to a high specific activity gamma emitting isotope of iodine. MCF-7 human breast cancer cells were incubated with 16..cap alpha..-(/sup 125/I)iodoestradiol alone ((/sup 125/I)E/sub 2/), or with 16..cap alpha..-(/sup 125/I)iodoestradiol plus 100-fold excess 17..beta..-estradiol (E/sub 2/), and then viably frozen. After 8 weeks, the (/sup 125/I)E/sub 2/ exposed cells and the (/sup 125/I)E/sub 2/ plus E/sub 2/ exposed cells were 10% and 77% of controls, respectively. When the breast cancer cell line MDA-MB-231 which does not contain estrogen receptors was used, the rate of cell death was similar to the competed MCF-7 cells. When specific cytotoxicity was compared using a cloning technique, nearly a 5 log reduction in surviving cell fraction was seen with (/sup 125/I)E/sub 2/, as compared to identical cells treated with (/sup 125/I)E/sub 2/ plus competitor. This technique shows promise for selecting a population of cells with defects in their estrogen receptor and in studying subcellular hormone interactions.

• OSTI ID: 7086126
• Journal Information: Endocrinology; (United States), Journal Name: Endocrinology; (United States) Vol. 110:6; ISSN ENDOA
• Country of Publication: United States
• Language: English

Related Subjects

550604 -- Medicine-- Unsealed Radionuclides in Therapy-- (1980-)
560121* -- Radiation Effects on Cells-- External Source-- (-1987)
62 RADIOLOGY AND NUCLEAR MEDICINE
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
ANIMAL CELLS
BETA DECAY RADIOISOTOPES
BIOLOGICAL EFFECTS
BIOLOGICAL RADIATION EFFECTS
BODY
CELL KILLING
DAYS LIVING RADIOISOTOPES
ELECTRON CAPTURE RADIOISOTOPES
ESTRADIOL
ESTRANES
ESTROGENS
GLANDS
HORMONES
HYDROXY COMPOUNDS
INTERMEDIATE MASS NUCLEI
IODINE 125
IODINE ISOTOPES
ISOTOPE APPLICATIONS
ISOTOPES
LABELLED COMPOUNDS
MAMMARY GLANDS
NUCLEI
ODD-EVEN NUCLEI
ORGANIC COMPOUNDS
ORGANIC HALOGEN COMPOUNDS
ORGANIC IODINE COMPOUNDS
ORGANS
RADIATION EFFECTS
RADIOINDUCTION
RADIOISOTOPES
RECEPTORS
STEROID HORMONES
STEROIDS
TRACER TECHNIQUES
TUMOR CELLS