Specific cytotoxicity of 16 alpha-(/sup 125/I)iodoestradiol for estrogen receptor-containing breast cancer cells

Journal Article · Tue Jun 01 04:00:00 EDT 1982 · Endocrinology; (United States)

OSTI ID:6783032

Bronzert, D A; Hochberg, R B; Lippman, M E

We attempted to induce specific killing of estrogen receptor-containing breast cancer cells using estradiol coupled to a high specific activity gamma emitting isotope of iodine. MCF-7 human breast cancer cells were incubated with 16 alpha-(/sup 125/I)iodoestradiol alone ((/sup 125/I)E2), or with 16 alpha-(/sup 125/I)iodoestradiol plus 100-fold excess 17 beta-estradiol (E2), and then viably frozen. After 8 weeks, the (/sup 125/I)E2 exposed cells and the (/sup 125/I)E2 plus E2 exposed cells were 10% and 77% of controls, respectively. When the breast cancer cell line MDA-MB-231 which does not contain estrogen receptors was used, the rate of cell death was similar to the competed MCF-7 cells. When specific cytotoxicity was compared using a cloning technique, nearly a 5 log reduction in surviving cell fraction was seen with (/sup 125/I)E2, as compared to identical cells treated with (/sup 125/I)E2 plus competitor. This technique shows promise for selecting a population of cells with defects in their estrogen receptor and in studying subcellular hormone interactions.

• Research Organization: National Cancer Institute, Bethesda, Maryland
• OSTI ID: 6783032
• Journal Information: Endocrinology; (United States), Journal Name: Endocrinology; (United States) Vol. 110:6; ISSN ENDOA
• Country of Publication: United States
• Language: English

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62 RADIOLOGY AND NUCLEAR MEDICINE
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
ANIMAL CELLS
BETA DECAY RADIOISOTOPES
BIOCHEMISTRY
BODY
CARCINOMAS
CELL KILLING
CHEMISTRY
DAYS LIVING RADIOISOTOPES
DISEASES
ELECTRON CAPTURE RADIOISOTOPES
ESTRADIOL
ESTRANES
ESTROGENS
GLANDS
HORMONES
HYDROXY COMPOUNDS
INTERMEDIATE MASS NUCLEI
INTERNAL IRRADIATION
IODINE 125
IODINE ISOTOPES
IRRADIATION
ISOTOPE APPLICATIONS
ISOTOPES
MAMMARY GLANDS
MEDICINE
NEOPLASMS
NUCLEAR MEDICINE
NUCLEI
ODD-EVEN NUCLEI
ORGANIC COMPOUNDS
ORGANS
PATIENTS
PHYSIOLOGY
RADIOISOTOPES
RADIOLOGY
RADIOSENSITIVITY
RADIOTHERAPY
RECEPTORS
STEROID HORMONES
STEROIDS
THERAPY
TUMOR CELLS