Inhibition of semiconservative DNA synthesis in ICR 2A frog cells exposed to monochromatic uv wavelengths (252-313 nm) and photoreactivating light

Journal Article · Tue Jun 01 04:00:00 EDT 1982 · Radiat. Res.; (United States)

DOI:https://doi.org/10.2307/3575728· OSTI ID:7086102

Rosenstein, B S

Exposure of ICR 2A frog cells to monochromatic uv wavelengths in the range 252-313 nm caused an inhibition of semiconservative DNA synthesis which was partially relieved in cells receiving a post irradiation treatment with photoreactivating light (>350 nm). Hence pyrimidine dimers acted as lesions blocking DNA synthesis in uv-irradiated cells based upon the specificity of photoreactivating enzyme for the light-dependent monomerization of dimers in DNA. Compared with the shorter wavelengths tested, however, this recovery of DNA synthesis was not as great in cells exposed to 302-nm radiation and was nearly absent in 313-nm-irradiated cells up to 12 hr after treatment. These results suggest that nondimer photoproducts also play an important role in causing DNA synthesis inhibition in cells exposed to wavelengths greater than 300 nm.

• Research Organization: Univ. of Texas Health Science Center, Dallas
• OSTI ID: 7086102
• Journal Information: Radiat. Res.; (United States), Journal Name: Radiat. Res.; (United States) Vol. 90:3; ISSN RAREA
• Country of Publication: United States
• Language: English

Related Subjects

560114 -- Radiation Effects on Biochemicals-- In Animals-- (-1987)
560121* -- Radiation Effects on Cells-- External Source-- (-1987)
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
AMPHIBIANS
ANIMAL CELLS
ANIMALS
AQUATIC ORGANISMS
BIOLOGICAL EFFECTS
BIOLOGICAL RADIATION EFFECTS
BIOLOGICAL RECOVERY
BIOLOGICAL REPAIR
BIOSYNTHESIS
DIMERS
DNA
ELECTROMAGNETIC RADIATION
FROGS
INHIBITION
MONOCHROMATIC RADIATION
NUCLEIC ACIDS
ORGANIC COMPOUNDS
PHOTOREACTIVATION
RADIATION EFFECTS
RADIATIONS
RECOVERY
REPAIR
SYNTHESIS
ULTRAVIOLET RADIATION
VERTEBRATES