DNA repair in normal human and xeroderma pigmentosum group A fibroblasts following treatment with various methanesulfonates and the demonstration of a long-patch repair component

Journal Article · Fri Jan 01 04:00:00 EST 1982 · Carcinogenesis (N.Y.); (United States)

DOI:https://doi.org/10.1093/carcin/3.1.7· OSTI ID:7084487

Snyder, R D; Regan, J D

Excision repair of DNA in normal and xeroderma pigmentosum complementation group A fibroblasts were examined following treatment with methyl-, ethyl-, and isopropyl methanesulfonate. Studies utilizing repair synthesis methods and inhibition with arabinofuranosyl cytosine revealed two distinct phases of repair; one commencing and terminating within the first 3-5 h after the treatment, and a second much longer phase extending from 9-35 h post-treatment. Both phases of repair have a long-patch component, which establishes for the first time the existence of this mode of repair in response to alkane sulfonate damage. While xeroderma cells display somewhat fewer alkaline labile sites in their DNA following alkylation treatment than do their normal counterparts, researchers are unable to demonstrate a deficiency of these cells in either of the two phases of repair.

• Research Organization: University of Tennessee, Oak Ridge Graduate School of Biomedical Science, Oak Ridge, TN
• OSTI ID: 7084487
• Journal Information: Carcinogenesis (N.Y.); (United States), Journal Name: Carcinogenesis (N.Y.); (United States) Vol. 3:1; ISSN CRNGD
• Country of Publication: United States
• Language: English

Related Subjects

550400 -- Genetics
560301* -- Chemicals Metabolism & Toxicology-- Cells-- (-1987)
59 BASIC BIOLOGICAL SCIENCES
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
ALKANES
ALKYLATION
ANIMAL CELLS
BIOLOGICAL EFFECTS
BIOLOGICAL RECOVERY
BIOLOGICAL REPAIR
CHEMICAL REACTIONS
COMPARATIVE EVALUATIONS
CONNECTIVE TISSUE CELLS
DECOMPOSITION
DISEASES
DNA
FIBROBLASTS
GENETIC EFFECTS
HYDROCARBONS
METHANE
NUCLEIC ACIDS
ORGANIC COMPOUNDS
ORGANIC SULFUR COMPOUNDS
PHOTOCHEMICAL REACTIONS
PHOTOLYSIS
RECOVERY
REPAIR
SKIN DISEASES
SOMATIC CELLS
SULFONATES
TIME DEPENDENCE
XERODERMA PIGMENTOSUM