Inactivation of ultraviolet repair in normal and xeroderma pigmentosum cells by methyl methanesulfonate
Journal Article
·
· Cancer Res.; (United States)
OSTI ID:5363906
Excision repair of ultraviolet damage in the DNA of normal and xeroderma pigmentosum (Groups C, D, and variant) cells was inactivated by exposure of cells to methyl methanesulfonate immediately before irradiation independent of the presence of 0 to 10% fetal calf serum. The inactivation could be represented by a semilog relationship between the amount of repair and methyl methanesulfonate concentration up to approximately 5 mM. The inactivation can be considered to occur as the result of alkylation of a large (about 10(6) daltons) repair enzyme complex, and the dose required to reduce repair to 37% for most cells types was between 4 and 7 mM. No consistent, large difference in sensitivity to methyl methanesulfonate was found in any xeroderma pigmentosum complementation group compared to normal cells, implying that reduced repair in these groups may be caused by small inherited changes in the amino acid composition (i.e., point mutations or small deletions) rather than by losses of major components of the repair enzyme complex.
- Research Organization:
- Laboratory of Radiobiology and Environmental Health, University of California, San Francisco
- OSTI ID:
- 5363906
- Journal Information:
- Cancer Res.; (United States), Journal Name: Cancer Res.; (United States) Vol. 42:3; ISSN CNREA
- Country of Publication:
- United States
- Language:
- English
Similar Records
Regulation of DNA repair in serum-stimulated xeroderma pigmentosum cells
DNA repair in normal human and xeroderma pigmentosum group A fibroblasts following treatment with various methanesulfonates and the demonstration of a long-patch repair component
Repair of uv-damaged DNA in normal human and xeroderma pigmentosum cells
Journal Article
·
Mon Oct 01 00:00:00 EDT 1984
· J. Cell Biol.; (United States)
·
OSTI ID:6099224
DNA repair in normal human and xeroderma pigmentosum group A fibroblasts following treatment with various methanesulfonates and the demonstration of a long-patch repair component
Journal Article
·
Thu Dec 31 23:00:00 EST 1981
· Carcinogenesis (N.Y.); (United States)
·
OSTI ID:7084487
Repair of uv-damaged DNA in normal human and xeroderma pigmentosum cells
Journal Article
·
Sun Dec 31 23:00:00 EST 1972
· Stud. Biophys., v. 36-37, pp. 375-386
·
OSTI ID:4424056
Related Subjects
560111 -- Radiation Effects on Biochemicals-- In Vitro-- (-1987)
560114* -- Radiation Effects on Biochemicals-- In Animals-- (-1987)
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
ANIMAL CELLS
ANIMALS
BIOLOGICAL EFFECTS
BIOLOGICAL RECOVERY
BIOLOGICAL REPAIR
BODY
CELL CULTURES
CHEMICAL REACTIONS
DISEASES
DNA
ELECTROMAGNETIC RADIATION
ESTERS
MAMMALS
MAN
METHYL METHANESULFONATE
MUTAGENS
NEOPLASMS
NUCLEIC ACIDS
ORGANIC COMPOUNDS
ORGANIC SULFUR COMPOUNDS
ORGANS
PHOTOCHEMICAL REACTIONS
PRIMATES
RADIATIONS
RECOVERY
REPAIR
RESPONSE MODIFYING FACTORS
SENSITIVITY
SKIN
SKIN DISEASES
SULFONIC ACID ESTERS
TOXICITY
ULTRAVIOLET RADIATION
VERTEBRATES
XERODERMA PIGMENTOSUM
XP CELLS
560114* -- Radiation Effects on Biochemicals-- In Animals-- (-1987)
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
ANIMAL CELLS
ANIMALS
BIOLOGICAL EFFECTS
BIOLOGICAL RECOVERY
BIOLOGICAL REPAIR
BODY
CELL CULTURES
CHEMICAL REACTIONS
DISEASES
DNA
ELECTROMAGNETIC RADIATION
ESTERS
MAMMALS
MAN
METHYL METHANESULFONATE
MUTAGENS
NEOPLASMS
NUCLEIC ACIDS
ORGANIC COMPOUNDS
ORGANIC SULFUR COMPOUNDS
ORGANS
PHOTOCHEMICAL REACTIONS
PRIMATES
RADIATIONS
RECOVERY
REPAIR
RESPONSE MODIFYING FACTORS
SENSITIVITY
SKIN
SKIN DISEASES
SULFONIC ACID ESTERS
TOXICITY
ULTRAVIOLET RADIATION
VERTEBRATES
XERODERMA PIGMENTOSUM
XP CELLS