Simplified method for DNA and protein staining of human hematopoietic cell samples

Conference · Mon Dec 31 23:00:00 EST 1979

DOI:https://doi.org/10.1002/cyto.990020204· OSTI ID:7079907

Crissman, H A; Egmond, J V; Holdrinet, R S; Pennings, A; Haanen, C

A rapid reproducible method yielding high resolution analysis of DNA and protein in human hematopoietic cell samples was developed by modification of the propidium iodide (PI) and fluorescein isothiocyanate (FITC) procedure. Cell staining involved sequential addition of each reagent (RNase, FITC, and PI) to ethanol-fixed cells and requires no centrifiguation steps. Stained cells are analyzed in the reagent solutions. Analysis of bone marrow samples from multiple myeloma patients revealed mixed 2C DNA and aneuploid populations with the aneuploid cells having a significantly higher protein content. This approach permitted differential cell cycle kinetic analysis of the 2C DNA and the aneuploid population.

• Research Organization: Los Alamos Scientific Lab., NM (USA)
• DOE Contract Number: W-7405-ENG-36
• OSTI ID: 7079907
• Report Number(s): LA-UR-80-3389; CONF-801219-1
• Country of Publication: United States
• Language: English

Related Subjects

550300* -- Cytology
59 BASIC BIOLOGICAL SCIENCES
ANIMAL CELLS
ANIMALS
AROMATICS
BONE MARROW CELLS
CARBOXYLIC ACIDS
CELL CYCLE
CELL FLOW SYSTEMS
CONNECTIVE TISSUE CELLS
CYTOLOGICAL TECHNIQUES
DNA
DYES
FLUORESCEIN
HYDROXY ACIDS
HYDROXY COMPOUNDS
ISOTHIOCYANATES
MAMMALS
MAN
NUCLEIC ACIDS
ORGANIC ACIDS
ORGANIC COMPOUNDS
ORGANIC HALOGEN COMPOUNDS
ORGANIC IODINE COMPOUNDS
ORGANIC SULFUR COMPOUNDS
PHENOLS
PLOIDY
POLYPHENOLS
PRIMATES
PROTEINS
SOMATIC CELLS
STAINS
VERTEBRATES