Simplified method for DNA and protein staining of human hematopoietic cell samples. [Cell flow systems]

Journal Article · Wed Dec 31 23:00:00 EST 1980 · Cytometry (Baltimore); (United States)

OSTI ID:5348342

Crissman, H A ^[1]; Egmond, J V; Holdrinet, R S; Pennings, A; Haanen, C

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1. Los Alamos National Lab., NM

A rapid reproducible method yielding high resolution analysis of DNA and protein in human hematopoietic cell samples has been developed by modification of the propidium iodide and fluorescein isothiocyanate procedure. Cell staining involves sequential addition of each reagent (RNase, fluorescein isothiocyanate and propidium iodide) to ethanol-fixed cells and requires no centrifugation steps. Stained cells are analyzed in the reagent solutions. Analysis of bone marrow samples from multiple myeloma patients showed mixed normal and aneuploid populations with a major portion of the aneuploid cells having a significantly higher protein content. This approach permitted differential cell cycle analysis of normal and the aneuploid populations.

• OSTI ID: 5348342
• Journal Information: Cytometry (Baltimore); (United States), Journal Name: Cytometry (Baltimore); (United States) Vol. 2:2; ISSN CYTOD
• Country of Publication: United States
• Language: English

Related Subjects

550200* -- Biochemistry
550300 -- Cytology
59 BASIC BIOLOGICAL SCIENCES
ANEUPLOIDY
ANIMAL CELLS
BLOOD FORMATION
BONE MARROW CELLS
CELL CYCLE
CELL FLOW SYSTEMS
CHEMICAL ANALYSIS
CONNECTIVE TISSUE CELLS
DIAGNOSTIC USES
DISEASES
DNA
NEOPLASMS
NUCLEIC ACIDS
ORGANIC COMPOUNDS
PATIENTS
PHOTOMETRY
PLOIDY
PROTEINS
SOMATIC CELLS
USES