Assignment of the gene for the core protein II (UQCRC2) subunit of the mitochondrial cytochrome bc[sub 1] complex to human chromosome 16p12
Journal Article
·
· Genomics; (United States)
- Queen's Univ., Kingston (Canada) Kingston General Hospital (Canada)
- Univ. of Nagoya (Japan)
- McGill Univ., Montreal (Canada) Montreal Children's Hospital (Canada)
The mammalian cytochrome be[sub 1] complex (complex III) of the mitochondrial respiratory chain catalyzes electron transfer from ubiquinol to cytochrome c. The complex consists of 10-11 subunits: Core proteins I and II, cytochromes b and c[sub 1], the Rieske iron-sulfur protein, the ubiquinone-binding protein, the hinge protein, and 3-4 subunits of low molecular weight. Cytochrome b is encoded by the mitochondrial genome; the other subunits are encoded by nuclear genes. Both the human cytochrome c[sub 1] and the human ubiquinone-binding protein subunits have been assigned to chromosome 8 by somatic cell hybrid mapping. In this study, the authors used in situ hybridization to map core protein II. In situ hybridization to BrdU-synchronized peripheral blood lymphocytes was performed using the method of Harper and Saunders. Chromosomes were stained with a modified fluorescence, 0.25% Wright's stain procedure. The positions of silver grains directly over or touching well-banded metaphase chromosomes were mapped to an ISCN idiogram. The analysis of the distribution of 200 silver grams following in situ hybridization revealed a significant clustering of grains in the p12 region of chromosome 16. The assignment of the core II subunit to human chromosome 16p12 confirms that it is encoded by the nuclear, rather than the mitochondrial, genome. The identification of a single strong hybridization signal is indicative of one locus with no pseudogenes. 6 refs., 1 fig.
- OSTI ID:
- 7077691
- Journal Information:
- Genomics; (United States), Journal Name: Genomics; (United States) Journal Issue: 2 Vol. 18:2; ISSN GNMCEP; ISSN 0888-7543
- Country of Publication:
- United States
- Language:
- English
Similar Records
Assignment of the gene for the cytochrome c[sub 1] subunit of the mitochondrial cytochrome bc[sub 1] complex (CYC1) to human chromosome 8q24. 3
Assignment of the gene (UQCRFS1) for the Rieske iron-sulfur protein subunit of the mitochondrial cytochrome bc[sub 1] complex to the 22q13 and 19q12-q13. 1 regions of the human genome
Assignment of the endothelial-specific protein receptor tyrosine kinase gene (TEK) to human chromosome 9p21
Journal Article
·
Fri Jan 14 23:00:00 EST 1994
· Genomics; (United States)
·
OSTI ID:7160440
Assignment of the gene (UQCRFS1) for the Rieske iron-sulfur protein subunit of the mitochondrial cytochrome bc[sub 1] complex to the 22q13 and 19q12-q13. 1 regions of the human genome
Journal Article
·
Sun May 01 00:00:00 EDT 1994
· Genomics; (United States)
·
OSTI ID:7029711
Assignment of the endothelial-specific protein receptor tyrosine kinase gene (TEK) to human chromosome 9p21
Journal Article
·
Thu Sep 15 00:00:00 EDT 1994
· Genomics
·
OSTI ID:255155