Production of ethanol from biopolymers by anaerobic, thermophilic, and extreme thermophilic bacteria. III. Thermoanaerobacter ethanolicus JW200 and its mutants in batch cultures and resting cell experiments

Conference · Sat Jan 01 04:00:00 EST 1983 · Biotechnol. Bioeng. Symp.; (United States)

OSTI ID:7077526

Wiegel, J; Carreira, L H; Mothershed, C P; Puls, J

Several thermophilic and extreme thermophilic anaerobic bacteria can utilize hemicellulose (xylan polymer) from birch- and beechwood directly. Thermoanaerobacter ethanolicus JW200 exhibited the highest ethanol formation, although the extracellular xylanase and xylosidase activities were very low. All bacteria rapidly utilized xylose before the polymers were utilized at a lower rate. With resting cell suspensions of T. ethanolicus and its mutants, the ethanol formation rates were as high as 60 mmol (2.76 g) and 30 mmol (1.3 g) ethanol per liter per hour from glucose and xylose, respectively. After 1 hour the ethanol productions in the concentrated cell suspensions were linear for over 10 hours from glucose or xylose; however, with soluble starch (DE 10) the rates were increasing with time. From these experiments it is concluded that the contiuous culture experiments with hemicellulosic material and/or starch have to be performed with recycling of the cells and the extracellular enzymes. 25 references, 6 figures, 3 tables.

• Research Organization: Univ. of Georgia, Athens
• DOE Contract Number: AS09-79ER10499
• OSTI ID: 7077526
• Report Number(s): CONF-830567-
• Conference Information: Journal Name: Biotechnol. Bioeng. Symp.; (United States) Journal Volume: 13
• Country of Publication: United States
• Language: English

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59 BASIC BIOLOGICAL SCIENCES
ALCOHOLS
ALDEHYDES
BACTERIA
BEECH TREES
BIOCHEMICAL REACTION KINETICS
BIOCONVERSION
BIRCHES
CARBOHYDRATES
CHEMICAL REACTIONS
CLOSTRIDIUM
CLOSTRIDIUM THERMOCELLUM
CLOSTRIDIUM THERMOSACCHAROLYTICUM
COMPARATIVE EVALUATIONS
DECOMPOSITION
ETHANOL
FERMENTATION
GLUCOSE
GROWTH
HEMICELLULOSE
HEXOSES
HYDROXY COMPOUNDS
KINETICS
MICROORGANISMS
MONOSACCHARIDES
MUTANTS
ORGANIC COMPOUNDS
PENTOSES
PLANTS
POLYSACCHARIDES
PRODUCTION
REACTION KINETICS
REAGENTS
SACCHARIDES
STARCH
TREES
XYLANS
XYLOSE