Analysis of the role of recombination and repair in mutagenesis of Escherichia coli by uv irradiation
Multiple mutant strains have been tested for their mimicry of the uv-mutagenesis deficiency of a recA single mutant. Revertants to histidine prototrophy and clear plaque mutants of lambda were scored to determine capacity for uv-mutagenesis. Nearly normal capacity was shown by a uvr/sup +/ recB/sup -/ recF/sup -/ strain, which shows almost no recA-dependent recombination, by uvr/sup -/ recB/sup +/ recF/sup -/ strains, which show almost no recA-dependent repair and by a uvrA/sup -/ recB/sup -/ recF/sup -/ strain, which shows neither recA-dependent recombination nor repair. Since the uvr mutants can be assumed to show additionally no excision repair, these results may mean the uv-mutagenesis occurs during processes other than recombination and repair. Alternative hypotheses are discussed. The slight difference in mutagenic capacity was traced to the recF single mutation, which blocks the production of unmixed bursts of clear-plaque lambda mutants. Since this accounts for only about 10% of the mutations leading to clear-plaque mutants, it is suggested that there is more than one uv-mutagenic process.
- Research Organization:
- Univ. of California, Berkeley
- OSTI ID:
- 7075755
- Journal Information:
- Genetics; (United States), Journal Name: Genetics; (United States) Vol. 87:1; ISSN GENTA
- Country of Publication:
- United States
- Language:
- English
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Related Subjects
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
BACTERIA
BIOLOGICAL EFFECTS
BIOLOGICAL RADIATION EFFECTS
BIOLOGICAL RECOVERY
BIOLOGICAL REPAIR
ELECTROMAGNETIC RADIATION
ESCHERICHIA COLI
GENE RECOMBINATION
MICROORGANISMS
MUTAGENESIS
MUTATIONS
RADIATION EFFECTS
RADIATIONS
RADIOINDUCTION
RECOVERY
REPAIR
ULTRAVIOLET RADIATION