Autoradiographic identification of ornithine decarboxylase in mouse kidney by means of alpha-(5-/sup 14/C)difluoromethylornithine
alpha-Difluoromethylornithine is an enzyme-activated irreversible inhibitor of ornithine decarboxylase that forms a covalent bond with the enzyme. When alpha-(5-/sup 14/C)difluoromethylornithine was administered to androgen-treated mice, only ornithine decarboxylase became labeled. Autoradiographic examination of kidney, liver, and brain indicated much more extensive incorporation of labeled difluoromethylornithine into kidney protein than into the protein of the other tissues. Such incorporation was greatly reduced by prior treatment of the mice with cycloheximide. These results correlate with the presence of ornithine decarboxylase activity which is much higher in the kidney than in the other tissues and is lost rapidly when protein synthesis is inhibited. The binding of this drug in vivo, therefore, is useful for determining the distribution of ornithine decarboxylase. The enzyme was predominantly located in the proximal tubule cells of the kidney in androgen-treated mice.
- Research Organization:
- Department of Physiology, Pennsylvania State University College of Medicine, Hershey
- OSTI ID:
- 7071935
- Journal Information:
- Science (Washington, D.C.); (United States), Vol. 217:4554
- Country of Publication:
- United States
- Language:
- English
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Related Subjects
CARBON 14 COMPOUNDS
ISOTOPE APPLICATIONS
DECARBOXYLASES
ENZYME ACTIVITY
ANDROGENS
AUTORADIOGRAPHY
BIOCHEMICAL REACTION KINETICS
CYCLOHEXIMIDE
ENZYME INHIBITORS
KIDNEYS
MICE
ORNITHINE
PHYSIOLOGY
AMINO ACIDS
ANDROSTANES
ANIMALS
ANTI-INFECTIVE AGENTS
ANTIBIOTICS
BODY
CARBON-CARBON LYASES
CARBOXY-LYASES
CARBOXYLIC ACIDS
DRUGS
ENZYMES
FUNGICIDES
HORMONES
KINETICS
LABELLED COMPOUNDS
LYASES
MAMMALS
ORGANIC ACIDS
ORGANIC COMPOUNDS
ORGANS
PESTICIDES
REACTION KINETICS
RODENTS
STEROID HORMONES
STEROIDS
VERTEBRATES
550201* - Biochemistry- Tracer Techniques
551001 - Physiological Systems- Tracer Techniques