Autoradiographic identification of ornithine decarboxylase in mouse kidney by means of. cap alpha. -(5-/sup 14/C)difluoromethylornithine
..cap alpha..-Difluoromethylornithine is an enzyme-activated irreversible inhibitor of ornithine decarboxylase that forms a covalent bond with the enzyme. When ..cap alpha..-(5-/sup 14/C)difluoromethylornithine was administered to androgen-treated mice, only ornithine decarboxylase became labeled. Autoradiographic examination of kidney, liver, and brain indicated much more extensive incorporation of labeled difluoromethylornithine into kidney protein than into the protein of the other tissues. Such incorporation was greatly reduced by prior treatment of the mice with cycloheximide. These results correlate with the presence of ornithine decarboxylase activity which is much higher in the kidney than in the other tissues and is lost rapidly when protein synthesis is inhibited. The binding of this drug in vivo, therefore, is useful for determining the distribution of ornithine decarboxylase. The enzyme was predominantly located in the proximal tubule cells of the kidney in androgen-treated mice.
- Research Organization:
- Pennsylvania State Univ., Hershey
- OSTI ID:
- 5053997
- Journal Information:
- Science (Washington, D.C.); (United States), Vol. 217
- Country of Publication:
- United States
- Language:
- English
Similar Records
cap alpha. -Difluoromethylornithine, an irreversible inhibitor of ornithine decarboxylase, inhibits tumor promoter-induced polyamine accumulation and carcinogenesis in mouse skin
Production and characterization of monoclonal antibody against mammalian ornithine decarboxylase
Related Subjects
CARBON 14 COMPOUNDS
TRACER TECHNIQUES
DECARBOXYLASES
ENZYME ACTIVITY
ENZYME INHIBITORS
BIOCHEMISTRY
ANDROGENS
AUTORADIOGRAPHY
BIOLOGICAL LOCALIZATION
BRAIN
KIDNEYS
LIVER
MICE
ORNITHINE
AMINO ACIDS
ANDROSTANES
ANIMALS
BODY
CARBON-CARBON LYASES
CARBOXY-LYASES
CARBOXYLIC ACIDS
CENTRAL NERVOUS SYSTEM
CHEMISTRY
DIGESTIVE SYSTEM
ENZYMES
GLANDS
HORMONES
ISOTOPE APPLICATIONS
LABELLED COMPOUNDS
LYASES
MAMMALS
NERVOUS SYSTEM
ORGANIC ACIDS
ORGANIC COMPOUNDS
ORGANS
RODENTS
STEROID HORMONES
STEROIDS
VERTEBRATES
550201* - Biochemistry- Tracer Techniques
551001 - Physiological Systems- Tracer Techniques