Contribution of depressed reuptake to the depletion of norepinephrine from rat heart and spleen during endotoxin shock

Journal Article · Fri Jan 01 00:00:00 EST 1982 · Circ. Shock; (United States)

OSTI ID:7071739

Pardini, B J; Jones, S B; Filkins, J P

Norepinephrine content (microgram/g) was depressed in hearts and spleens of fasted male Holtzman rats treated intravenously with Salmonella enteritidis lipopolysaccharide (14-17 mg/kg). To investigate the mechanism of norepinephrine depletion during endotoxicosis, in vivo norepinephrine reuptake was evaluated in control and severely shocked rats using the incorporation of /sup 3/H-norepinephrine into hearts and spleens. Incorporation of /sup 3/H-norepinephrine into spleens of endotoxic rats was reduced 88%. In contrast, cardiac tissue incorporation of /sup 3/H-norepinephrine was not significantly impaired. In vitro analysis of total norepinephrine retained in cardiac and splenic tissue slices incubated with /sup 3/H-norepinephrine yielded results consistent with in vivo experiments: Splenic norepinephrine reuptake was significantly decreased on the order of 50% in preparations from endotoxic rats, while myocardial norepinephrine reuptake was the same in both groups. The results indicate that depression of norepinephrine reuptake is a mechanism of norepinephrine depletion in spleens but not hearts of endotoxic rats.

• Research Organization: Department of Physiology, Loyola University of Chicago, Stritch School of Medicine, Maywood, Illinois
• OSTI ID: 7071739
• Journal Information: Circ. Shock; (United States), Vol. 9:2
• Country of Publication: United States
• Language: English

Related Subjects

59 BASIC BIOLOGICAL SCIENCES
37 INORGANIC
ORGANIC
PHYSICAL AND ANALYTICAL CHEMISTRY
ADRENALINE
QUANTITATIVE CHEMICAL ANALYSIS
RATS
BIOLOGICAL SHOCK
COMPARATIVE EVALUATIONS
HEART
PHYSIOLOGY
SALMONELLA
SPLEEN
TOXICITY
TRITIUM COMPOUNDS
ADRENAL HORMONES
ANIMALS
AUTONOMIC NERVOUS SYSTEM AGENTS
BACTERIA
BODY
CARDIOTONICS
CARDIOVASCULAR AGENTS
CARDIOVASCULAR SYSTEM
CHEMICAL ANALYSIS
DRUGS
HORMONES
LABELLED COMPOUNDS
MAMMALS
MICROORGANISMS
NEUROREGULATORS
ORGANS
PATHOLOGICAL CHANGES
RODENTS
STEROID HORMONES
SYMPATHOMIMETICS
VERTEBRATES
550201* - Biochemistry- Tracer Techniques
551001 - Physiological Systems- Tracer Techniques
400102 - Chemical & Spectral Procedures