Radioisotopic assay of picomolar concentrations of coenzyme A in liver tissue
Journal Article
·
· Anal. Biochem.; (United States)
A single-step enzyme assay using (/sup 14/C)palmitic acid and bacterial acyl-coenzyme A synthetase (EC is described for the determination of reduced coenzyme A (CoASH) levels in liver samples. Use of this technique provides a rapid and accurate determination of CoASH in the range 1-250 pmol. Application of the method to the quantitation of CoASH in samples of human liver tissue and rat liver homogenate, isolated hepatocytes, and mitochondria is described.
- Research Organization:
- Flinders Univ. (Australia)
- OSTI ID:
- 7070068
- Journal Information:
- Anal. Biochem.; (United States), Journal Name: Anal. Biochem.; (United States) Vol. 168:1; ISSN ANBCA
- Country of Publication:
- United States
- Language:
- English
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Related Subjects
550201* -- Biochemistry-- Tracer Techniques
59 BASIC BIOLOGICAL SCIENCES
ALCOHOLS
ANIMALS
BODY
CARBON 14 COMPOUNDS
CARBOXYLIC ACIDS
CELL CONSTITUENTS
COENZYMES
DIGESTIVE SYSTEM
ENZYMES
GLANDS
GLYCOLS
HEXADECANOIC ACID
HYDROXY COMPOUNDS
LABELLED COMPOUNDS
LIGASES
LIVER
MAMMALS
MAN
MITOCHONDRIA
MONOCARBOXYLIC ACIDS
ORGANIC ACIDS
ORGANIC COMPOUNDS
ORGANIC POLYMERS
ORGANOIDS
ORGANS
POLYETHYLENE GLYCOLS
POLYMERS
PRIMATES
RADIOASSAY
RATS
RODENTS
VERTEBRATES
59 BASIC BIOLOGICAL SCIENCES
ALCOHOLS
ANIMALS
BODY
CARBON 14 COMPOUNDS
CARBOXYLIC ACIDS
CELL CONSTITUENTS
COENZYMES
DIGESTIVE SYSTEM
ENZYMES
GLANDS
GLYCOLS
HEXADECANOIC ACID
HYDROXY COMPOUNDS
LABELLED COMPOUNDS
LIGASES
LIVER
MAMMALS
MAN
MITOCHONDRIA
MONOCARBOXYLIC ACIDS
ORGANIC ACIDS
ORGANIC COMPOUNDS
ORGANIC POLYMERS
ORGANOIDS
ORGANS
POLYETHYLENE GLYCOLS
POLYMERS
PRIMATES
RADIOASSAY
RATS
RODENTS
VERTEBRATES