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Acetyl coenzyme A synthetase catalyzed reactions of coenzyme A with. cap alpha. -. beta. -unsaturated carboxylic acids

Journal Article · · Anal. Biochem.; (United States)
..cap alpha..,..beta..-Unsaturated coenzyme A(CoA) thioesters including acrylyl CoA, methacrylyl CoA, and propiolyl CoA were synthesized by catalysis with acetyl CoA synthetase. After isolation from the enzymatic reactions, the products were found to be the result of 1,4 addition of CoASH to the double bond and addition of water to the triple bond of the initial acyl CoA adducts. Structural determinations of these products by /sup 1/H NMR, /sup 13/C NMR, and the chemical reaction leading to their formation are described.
Research Organization:
Tufts Univ., Medford, MA (USA)
OSTI ID:
6768333
Journal Information:
Anal. Biochem.; (United States), Journal Name: Anal. Biochem.; (United States) Vol. 170:2; ISSN ANBCA
Country of Publication:
United States
Language:
English