Transphosphatidylation and base-exchange in the membranes of clostridium butyricum

Conference · Fri May 01 00:00:00 EDT 1987 · FASEB (Federation of American Societies for Experimental Biology) Journal; (USA)

OSTI ID:7068165

Walton, P A; Goldfine, H ^[1]

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1. Univ. of Pennsylvania, Philadelphia (USA)

Membrane particles from C. butyricum, incubated with phosphatidylserine (PS) and Triton X-100, produced three labelled phospholipids in addition to phosphatidylethanolamine (PE) formed by decarboxylation. These lipid were also formed when PE or phosphatidylglycerol (PG) was employed as substrate. 2D-TLC of intact lipids and their deacylation products showed that these lipids are phosphatidic acid, cardiolipid (CL), and previously unreported phosphatidyltriton (PT). Triton X-100 acts as both activator and substrate of the reaction. With radiolabel in different portions of the substrate phospholipid we demonstrated that the phosphatidyl moiety was transferred with the loss of the head-group in a phospholipase D-like manner. The acceptor molecule can be water or primary alcohols. Addition of exogenous unlabelled PG resulted in the formation of CL with concomitant decrease in the level of PT formed. Labelled PS, PE, and PG could also be formed upon addition of their corresponding alcoholic head group to incubations containing a labelled phosphatidyl donor and Triton X-100. These results indicate that, in C. butyricum, enzymic steps exists that would allow remodelling of the membrane phospholipids, without requiring de novo biosynthesis.

• OSTI ID: 7068165
• Report Number(s): CONF-870644--
• Journal Information: FASEB (Federation of American Societies for Experimental Biology) Journal; (USA), Journal Name: FASEB (Federation of American Societies for Experimental Biology) Journal; (USA) Vol. 46:6; ISSN FAJOE
• Country of Publication: United States
• Language: English

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59 BASIC BIOLOGICAL SCIENCES
ALCOHOLS
BACTERIA
BETA DECAY RADIOISOTOPES
BETA-MINUS DECAY RADIOISOTOPES
BIOSYNTHESIS
CARBOXYLESTERASES
CELL CONSTITUENTS
CELL MEMBRANES
CHEMICAL ACTIVATION
CHEMICAL REACTIONS
CLOSTRIDIUM
CLOSTRIDIUM BUTYRICUM
DAYS LIVING RADIOISOTOPES
DECARBOXYLATION
ENZYME ACTIVITY
ENZYMES
ESTERASES
ESTERS
HYDROLASES
HYDROXY COMPOUNDS
ISOTOPE APPLICATIONS
ISOTOPES
LABELLED COMPOUNDS
LIGHT NUCLEI
LIPASES
LIPIDS
MEMBRANES
MICROORGANISMS
NUCLEI
ODD-ODD NUCLEI
ORGANIC COMPOUNDS
ORGANIC PHOSPHORUS COMPOUNDS
PHOSPHOLIPIDS
PHOSPHORUS 32
PHOSPHORUS ISOTOPES
RADIOISOTOPES
SUBSTRATES
SYNTHESIS
TRACER TECHNIQUES