Control of bilayer stability in Clostridium butyricum: phase behavior of the ether lipids

Conference · Thu May 01 04:00:00 EDT 1986 · Fed. Proc., Fed. Am. Soc. Exp. Biol.; (United States)

OSTI ID:5269817

Goldfine, H; Johnston, N C; Mattai, J; Shipley, G G

In Clostridium butyricum approx. half of the phospholipid consists of phosphatidylethanolamine (PE), plasmenylethanolamine (PlaE), and the glycerol acetal of PlaE (GA). These lipids are predominantly in the outer monolayer of the cell membrane. Phosphatidylglycerol and cardiolipin appear to be predominantly in the inner monolayer. When grown on mixtures of palmitic (16:0) and oleic (18:1) acids in the absence of biotin, the 18:1 content of the phospholipid acyl chains responds strongly to the medium 18:1/16:0 ratio. At high 18:1/16:0, both the acyl and ether-linked chains are highly unsaturated, and the ratio of GA to PE plus PlaE increases from 0.7 to 2.0. /sup 31/P NMR and X-ray diffraction show that the oleate-enriched PE plus PlaE from C. butyricum grown on 100% oleate (PE/sub cb/o/) forms an H/sub 11/ phase above 0/sup 0/ C, whereas dioleoyl GA forms bilayers at physiological temperature X-ray diffraction studies with dioleoyl PE and dioleoyl GA show that 2:1 and 1:1 (wt/wt) ratios form mixed lamellar and hexagonal phases at 46/sup 0/ C. A 1:3 mixture, is lamellar at 46/sup 0/C. With PE/sub cb/o/dioleoyl GA, /sup 31/P NMR shows that a 2:1 mixture undergoes a lamellar to non-lamellar transition between 30 and 40/sup 0/C, whereas a 1:1 mixture forms a lamellar phase up to 45/sup 0/C. Growth of this organism with highly unsaturated membranes appears to depend on its ability to regulate its ether lipid composition, primarily by increasing the proportions of bilayer-forming GA.

• Research Organization: Univ. of Pennsylvania, Philadelphia
• OSTI ID: 5269817
• Report Number(s): CONF-8606151-
• Conference Information: Journal Name: Fed. Proc., Fed. Am. Soc. Exp. Biol.; (United States) Journal Volume: 45:6
• Country of Publication: United States
• Language: English

Related Subjects

550600 -- Medicine
550701* -- Microbiology-- Tracer Techniques
59 BASIC BIOLOGICAL SCIENCES
62 RADIOLOGY AND NUCLEAR MEDICINE
AMINES
BACTERIA
CARBOXYLIC ACIDS
CELL CONSTITUENTS
CELL MEMBRANES
CHEMICAL COMPOSITION
CLOSTRIDIUM
CLOSTRIDIUM BUTYRICUM
COHERENT SCATTERING
DIFFRACTION
ESTERS
GROWTH
ISOTOPES
LAMELLAE
LIGHT NUCLEI
LIPIDS
MEMBRANES
MICROORGANISMS
MONOCARBOXYLIC ACIDS
NMR SPECTRA
NUCLEI
ODD-EVEN NUCLEI
OLEIC ACID
ORGANIC ACIDS
ORGANIC COMPOUNDS
ORGANIC PHOSPHORUS COMPOUNDS
PHOSPHOLIPIDS
PHOSPHORUS 31
PHOSPHORUS ISOTOPES
SCATTERING
SPECTRA
STABLE ISOTOPES
X-RAY DIFFRACTION