Improvements in the methodology for analyzing receptor subtypes and neuronal populations affected by anticholinesterase exposure. Annual summary report, 15 November 1983-14 November 1984

Technical Report · Wed Nov 14 04:00:00 EST 1984

OSTI ID:7067822

Wamsley, J K

Conditions were defined that provide a means of selectively labeling subtypes of muscarinic receptors. The so-called M1 receptor population can be labeled with tritiated pirenzepine, while the receptor population labeled with tritiated quinuclidinyl benzilate (QNB) but not labeled with pirenzepine represents M2 receptor population. High- and low-affinity states of the receptors were also defined on the basis of agonist displacement of antagonist binding. Both the M1 and M2 receptor populations undergo axonal transport and the affinity states of these receptors are altered by neurochemical and neurosurgical lesions. Radioactive standards were developed that provide a means of quantitating the femtomoles of receptor bound with each ligand in microscopic regions of the brain. The technology was also devised to directly localize nicotinic cholinergic receptors using tritiated nicotine. It is now possible to localize several peptide receptors associated with cholinergic function including receptors for thyrotropin-releasing hormone (TRH) and somatostatin. The receptor autoradiographic technique was also carried beyond the receptor level of localization by using compounds to label adenylate cyclase and the GTP binding protein. This methodology should provide an elegant means of determining how anticholinesterase exposure has affected these many parameters of cholinergic nerve function.

• Research Organization: Utah Univ., Salt Lake City (USA). School of Medicine
• OSTI ID: 7067822
• Report Number(s): AD-A-191359/9/XAB
• Country of Publication: United States
• Language: English

Related Subjects

551001* -- Physiological Systems-- Tracer Techniques
59 BASIC BIOLOGICAL SCIENCES
ACETYLCHOLINE
AMINES
AMMONIUM COMPOUNDS
AUTONOMIC NERVOUS SYSTEM AGENTS
AUTORADIOGRAPHY
AZINES
BETA DECAY RADIOISOTOPES
BETA-MINUS DECAY RADIOISOTOPES
BIOASSAY
BODY
BRAIN
CARBOXYLIC ACIDS
CENTRAL NERVOUS SYSTEM
CHEMICAL BONDS
DISEASES
DOCUMENT TYPES
DRUGS
ESTERS
HETEROCYCLIC ACIDS
HETEROCYCLIC COMPOUNDS
HORMONES
HYDROGEN ISOTOPES
ISOTOPE APPLICATIONS
ISOTOPES
LABELLED COMPOUNDS
LIGHT NUCLEI
MICROSCOPY
NERVES
NERVOUS SYSTEM
NERVOUS SYSTEM DISEASES
NEUROREGULATORS
NICOTINIC ACID
NUCLEI
ODD-EVEN NUCLEI
ORGANIC ACIDS
ORGANIC COMPOUNDS
ORGANIC NITROGEN COMPOUNDS
ORGANS
PARASYMPATHOMIMETICS
PEPTIDE HORMONES
PROGRESS REPORT
PYRIDINES
QUATERNARY COMPOUNDS
RADIOACTIVITY
RADIOISOTOPES
STANDARDS
THYROID HORMONES
TRACER TECHNIQUES
TRITIUM
VITAMIN B GROUP
VITAMINS
YEARS LIVING RADIOISOTOPES