Effects of light on respiration and development of photosynthetic cells. Renewal application and progress report, March 1-November 1, 1980

Technical Report · Thu Nov 20 04:00:00 EST 1980

DOI:https://doi.org/10.2172/7065477· OSTI ID:7065477

Gibbs, M

The oxyhydrogen reaction in the presence and absence of CO/sub 2/ was studied in H/sub 2/- adapted Scenedesmus obliquus by monitoring the initial rates of H/sub 2/, O/sub 2/, and /sup 14/CO/sub 2/ uptake and the effect of inhibitors on these rates. Glucose and acetate respiration was competitive with H/sub 2/ uptake. KCN inhibited equally respiration and the oxyhydrogen reaction in the presence and absence of CO/sub 2/. It was concluded that the oxyhydrogen reaction both in the absence and presence of CO/sub 2/ has properties in common with components of respiration and photosynthesis. Participation of these two processes in the oxyhydrogen reaction would require a closely linked shuttle between mitochondrion and chloroplast. Protoplasts and chloroplasts will be isolated from a H/sub 2/-adapted alga in order to elucidate the cooperation between the two organelles. Acetate was shown to stimulate H/sub 2/ photoproduction in H/sub 2/-adapted algae even more so than an uncoupler of electron transport. The role of these compounds will be evaluated either in terms of the glyoxylate cycle or electron acceptors resulting in formation of alcohols. The term chloroplast respiration was proposed to account for the breakdown of polyglucan within the chloroplast. A means of reoxidizing reduced pyridine nucleotide was required to complete the cycle. A new enzyme ascorbic acid reduced pyridine nucleotide peroxidase was isolated from the chloroplast. The characterization of this enzyme will continue.

• Research Organization: Brandeis Univ., Waltham, MA (USA)
• DOE Contract Number: AS02-76ER03231
• OSTI ID: 7065477
• Report Number(s): DOE/ER/03231-5
• Country of Publication: United States
• Language: English

Related Subjects

550200* -- Biochemistry
550700 -- Microbiology
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59 BASIC BIOLOGICAL SCIENCES
ACETATES
ALGAE
BIOCHEMICAL REACTION KINETICS
BIOLOGICAL EFFECTS
CARBON COMPOUNDS
CARBON DIOXIDE
CARBON OXIDES
CARBOXYLIC ACID SALTS
CARBOXYLIC ACIDS
CELL CONSTITUENTS
CHALCOGENIDES
CHEMICAL REACTIONS
CHLOROPLASTS
ELECTRON TRANSFER
ENZYME INHIBITORS
ENZYMES
HETEROCYCLIC ACIDS
HETEROCYCLIC COMPOUNDS
HYDROGEN PRODUCTION
KINETICS
MICROORGANISMS
MITOCHONDRIA
ORGANIC ACIDS
ORGANIC COMPOUNDS
ORGANIC NITROGEN COMPOUNDS
ORGANOIDS
OXIDES
OXIDOREDUCTASES
OXYGEN COMPOUNDS
PEROXIDASES
PHOTOCHEMICAL REACTIONS
PHOTOSYNTHESIS
PLANTS
PORPHYRINS
REACTION KINETICS
RESPIRATION
SCENEDESMUS
SYNTHESIS
UNICELLULAR ALGAE