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Sister chromatid exchange induced by short-lived monoadducts produced by the bifunctional agents mitomycin C and 8-methoxypsoralen. [CHO cells]

Journal Article · · Environ. Mutagen.; (United States)
To see if DNA crosslinks are involved in the induction of sister chromated exchange (SCE), Chinese hamster ovary cells were exposed to two bifunctional alkylating agents,mitomycin C and 8-methoxypsoralen, and their monofunctional derivatives, decarbamoyl mitomycin C and angelicin. The data indicates that monoadducts, rather than crosslinks, are responsible for SCE formation. Furthermore, all agents but angelicin produced short-lived lesions that led to SCEs in the first period of DNA replication after treatment (twin SCEs). In contrast, angelicin, like methyl methanesulfonate and N-acetoxyacetylaminofluorene, produced lesions that lasted more than one cycle, indicating that several different types of DNA lesions are capable of SCE induction.
Research Organization:
Univ. of California, San Francisco
OSTI ID:
7065457
Journal Information:
Environ. Mutagen.; (United States), Journal Name: Environ. Mutagen.; (United States) Vol. 4:3; ISSN ENMUD
Country of Publication:
United States
Language:
English