Sister chromatid exchange induced by short-lived monoadducts produced by the bifunctional agents mitomycin C and 8-methoxypsoralen. [CHO cells]
To see if DNA crosslinks are involved in the induction of sister chromated exchange (SCE), Chinese hamster ovary cells were exposed to two bifunctional alkylating agents,mitomycin C and 8-methoxypsoralen, and their monofunctional derivatives, decarbamoyl mitomycin C and angelicin. The data indicates that monoadducts, rather than crosslinks, are responsible for SCE formation. Furthermore, all agents but angelicin produced short-lived lesions that led to SCEs in the first period of DNA replication after treatment (twin SCEs). In contrast, angelicin, like methyl methanesulfonate and N-acetoxyacetylaminofluorene, produced lesions that lasted more than one cycle, indicating that several different types of DNA lesions are capable of SCE induction.
- Research Organization:
- Univ. of California, San Francisco
- OSTI ID:
- 7065457
- Journal Information:
- Environ. Mutagen.; (United States), Vol. 4:3
- Country of Publication:
- United States
- Language:
- English
Similar Records
A new human photosensitive subject with a defect in the recovery of DNA synthesis after ultraviolet-light irradiation
Reduction of sister chromatid exchange frequency with time after mutagen exposure in Chinese hamster ovary cells in the presence of 3-aminobenzamide
Related Subjects
DNA
DAMAGE
MITOMYCIN
BIOLOGICAL EFFECTS
PSORALEN
SISTER CHROMATID EXCHANGES
BIOASSAY
ANIMAL CELLS
CROSS-LINKING
HAMSTERS
METABOLITES
METHOXY RADICALS
OVARIES
ALKOXY RADICALS
ANIMALS
ANTI-INFECTIVE AGENTS
ANTIBIOTICS
ANTICOAGULANTS
ANTIMITOTIC DRUGS
ANTINEOPLASTIC DRUGS
BODY
CHEMICAL REACTIONS
CHROMOSOMAL ABERRATIONS
COUMARINS
DRUGS
FEMALE GENITALS
GONADS
HEMATOLOGIC AGENTS
HETEROCYCLIC COMPOUNDS
MAMMALS
MUTATIONS
NUCLEIC ACIDS
ORGANIC COMPOUNDS
ORGANIC OXYGEN COMPOUNDS
ORGANS
POLYMERIZATION
RADICALS
RODENTS
VERTEBRATES
560301* - Chemicals Metabolism & Toxicology- Cells- (-1987)