Reduction of sister chromatid exchange frequency with time after mutagen exposure in Chinese hamster ovary cells in the presence of 3-aminobenzamide

Journal Article · Sat Dec 31 23:00:00 EST 1983 · Environ. Mutagen.; (United States)

DOI:https://doi.org/10.1002/em.2860060208· OSTI ID:6722382

Schwartz, J L; Morgan, W F; Wolff, S

3-aminobenzamide (3AB) is a potent inhibitor of poly(ADP-ribose) synthesis and has been reported to inhibit DNA repair. Inhibition of poly(ADP-ribose) synthesis by 3AB results in a potentiation of the cytotoxic effects of alkylating agents and synergistically increases the frequency of sister chromatid exchanges (SCEs) induced in Chinese hamster ovary cells by methyl methanesulfonate (MMS). Experiments were carried out to determine if this synergism was related to an inhibition of the repair or the removal of SCE-forming lesions by 3AB. 3AB appears to exert its effect only in cycling cells when bromodeoxyuridine is present. Therefore, the synergism between 3AB and alkylating agents in the induction of SCEs is unrelated to efects of 3AB on the repair or removal of SCE-forming lesions.

• Research Organization: Univ. of California, San Francisco
• OSTI ID: 6722382
• Journal Information: Environ. Mutagen.; (United States), Journal Name: Environ. Mutagen.; (United States) Vol. 6:2; ISSN ENMUD
• Country of Publication: United States
• Language: English

Related Subjects

550300 -- Cytology
560301* -- Chemicals Metabolism & Toxicology-- Cells-- (-1987)
59 BASIC BIOLOGICAL SCIENCES
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
ANIMAL CELLS
BIOLOGICAL RECOVERY
BIOLOGICAL REPAIR
CHO CELLS
CHROMOSOMAL ABERRATIONS
DNA
ENZYME INHIBITORS
ESTERS
METHYL METHANESULFONATE
MUTAGENS
MUTATION FREQUENCY
MUTATIONS
NITROSO COMPOUNDS
NUCLEIC ACIDS
ORGANIC COMPOUNDS
ORGANIC NITROGEN COMPOUNDS
ORGANIC SULFUR COMPOUNDS
RECOVERY
REPAIR
SISTER CHROMATID EXCHANGES
SULFONIC ACID ESTERS
SYNERGISM