Poliovirus retention in 75-cm soil cores after sewage and rainwater application

Journal Article · Mon Dec 01 04:00:00 EST 1980 · Appl. Environ. Microbiol.; (United States)

OSTI ID:7064815

Landry, E F; Vaughn, J M; Penello, W F

The adsorption rate of a guanidine-resistant strain of poliovirus LSc 2ab was measured in Long Island soils with in situ field cores (10.1 by 75 cm). The test virus was chosen because it exhibited soil adsorption and elution characteristics of a number of non-polioviruses. After the inoculation of cores with seeded sewage effluent at a 1-cm/h infiltration rate, cores were extracted, fractionated, and analyzed for total plaque-forming units per each 5-cm fraction. The results showed that 77% of the viruses were adsorbed in the first 5 cm of soil. An additional 11% were found in the 5- to 10-cm fraction, and a total of 96% of the viruses were adsorbed by 25 cm. The remaining 4% were uniformly distributed over the next 50 cm of soil, with a minimum of 0.23% in each soil section. Few viruses (< 0.22%) were observed in core filtrates. Analysis of the viral distribution pattern in seeded cores, after an application of a single rinse of either sewage effluent or rainwater, indicated that large-scale viral mobilization was absent. However, localized areas of viral movement were noted in both of the rinsed cores, with the rainwater rinsed cores exhibiting more extensive movement. All mobilized viruses were resorbed at lower core depths.

• Research Organization: Brookhaven National Lab., Upton, NY
• OSTI ID: 7064815
• Journal Information: Appl. Environ. Microbiol.; (United States), Journal Name: Appl. Environ. Microbiol.; (United States) Vol. 40:6; ISSN AEMID
• Country of Publication: United States
• Language: English

Related Subjects

510301 -- Environment
Terrestrial-- Radioactive Materials Monitoring &amp; Transport-- Soil-- (-1987)
54 ENVIRONMENTAL SCIENCES
550700* -- Microbiology
59 BASIC BIOLOGICAL SCIENCES
ADSORPTION
AFFINITY
BINDING ENERGY
BIOASSAY
CARBONIC ACID DERIVATIVES
CELL CULTURES
CHEMICAL ANALYSIS
COMPARATIVE EVALUATIONS
ENERGY
ENVIRONMENTAL EFFECTS
ENVIRONMENTAL TRANSPORT
GUANIDINES
HYDROGEN COMPOUNDS
MASS TRANSFER
MATHEMATICS
MICROORGANISMS
ORGANIC COMPOUNDS
ORGANIC NITROGEN COMPOUNDS
OXYGEN COMPOUNDS
PARASITES
PARTICLE SIZE
PARTICLES
POLIO VIRUS
QUANTITATIVE CHEMICAL ANALYSIS
RAIN WATER
RETENTION
SENSITIVITY
SEWAGE
SIZE
SOILS
SORPTION
STATISTICS
TOLERANCE
VIRUSES
WASTES
WATER