(Selected aspects of guard-cell biochemistry)
Synopsis of progress on current grant. The studies were proposed for five years. The proposal was funded for three years, and I am pleased to report that the proposed experiments have been conducted, in essence, although some protocols were altered as the work unfolded. We found that Abscisic acid (ABA) accumulates in guard cells before it does in other leaf cells during stress imposition. This is an important result that eliminates a long-standing objection to unequivocal assignment of a role to ABA in stress-related decrease in stomatal conductance. We also followed the kinetics of ABA following relief of stress. In a published manuscript, we report values for protoplasts. The results did not permit a conclusion as to the cellular source of ABA. We did not detect physiological isoforms and therefore did not search for phosphorylation or aggregation states. We have been successful in measuring plant cytoplasmic malate concentration, though not in Crassula, which presented intractable technical difficulties. These results lead me to the heretical general conclusion that malate inhibition is not the major single factor that regulates phosphoenolpyruvate. 48 refs., 8 figs.
- Research Organization:
- Florida State Univ., Tallahassee, FL (USA)
- Sponsoring Organization:
- DOE/ER
- DOE Contract Number:
- FG05-86ER13575
- OSTI ID:
- 7064466
- Report Number(s):
- DOE/ER/13575-4; ON: DE90015065
- Country of Publication:
- United States
- Language:
- English
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Related Subjects
550300 -- Cytology
59 BASIC BIOLOGICAL SCIENCES
ABSCISIC ACID
AUXINS
BIOCHEMICAL REACTION KINETICS
BIOCHEMISTRY
BIOLOGICAL ACCUMULATION
BIOLOGICAL EFFECTS
BIOLOGICAL STRESS
CARBOXYLIC ACIDS
CHEMICAL REACTIONS
CHEMISTRY
DOCUMENT TYPES
GENE REGULATION
KINETICS
LEAVES
MONOCARBOXYLIC ACIDS
ORGANIC ACIDS
ORGANIC COMPOUNDS
PHOSPHOENOLPYRUVATE
PHOSPHORYLATION
PHYSIOLOGY
PLANT CELLS
PLANT GROWTH REGULATORS
PROGRESS REPORT
REACTION KINETICS