(Guard cell biochemistry: Response to environmental stimuli causing changes in gas exchange): Progress report and introduction to experimental rationale and methodology

Technical Report · Wed Dec 31 23:00:00 EST 1986

OSTI ID:6177260

Procedures are described for microbiochemistry of individual guard cells. Specifically, studies are described for the determination of phosphoenolpyruvate carboxylase (PEPC) in single cells, electrophoresis of the protein complement of small number of cells, the cellular location of abscisic acid, measurement of the photosynthetically driven electron transport in guard cells, and the role of malate in regulation of PEPC. 19 figs., 1 tab. (DT)

• Research Organization: Florida State Univ., Tallahassee (USA)
• DOE Contract Number: FG05-86ER13575
• OSTI ID: 6177260
• Report Number(s): DOE/ER/13575-1; ON: DE87013193
• Country of Publication: United States
• Language: English

Related Subjects

09 BIOMASS FUELS
140504* -- Solar Energy Conversion-- Biomass Production & Conversion-- (-1989)
550501 -- Metabolism-- Tracer Techniques
59 BASIC BIOLOGICAL SCIENCES
ABSCISIC ACID
AUXINS
BACTERIA
BIOLOGICAL FUNCTIONS
CARBON 14 COMPOUNDS
CARBON-CARBON LYASES
CARBOXY-LYASES
CARBOXYLASE
CARBOXYLIC ACIDS
DISTRIBUTION
ELECTROPHORESIS
ENZYME ACTIVITY
ENZYMES
FUNCTIONS
HYDROXY ACIDS
ISOTOPE APPLICATIONS
LABELLED COMPOUNDS
LEAVES
LEGUMINOSAE
LYASES
MALIC ACID
MICROORGANISMS
MONOCARBOXYLIC ACIDS
ORGANIC ACIDS
ORGANIC COMPOUNDS
PLANT CELLS
PLANT GROWTH REGULATORS
PLANTS
RHIZOBIUM
SUBCELLULAR DISTRIBUTION
TRACER TECHNIQUES
VICIA