Site-directed mutagenesis of Azotobacter vinelandii ferredoxin I: (Fe-S) cluster-driven protein rearrangement

Journal Article · Mon Jan 01 04:00:00 EST 1990 · Proceedings of the National Academy of Sciences of the United States of America; (USA)

DOI:https://doi.org/10.1073/pnas.87.2.598· OSTI ID:7064361

Martin, A E; Burgess, B K ^[1]; Stout, C D ^[2]; Cash, V L; Dean, D R ^[3]; Jensen, G M; Stephens, P J ^[4]

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1. Univ. of California, Irvine (USA)
2. Research Institute of Scripps Clinic, La Jolla, CA (USA)
3. Virginia Polytechnic Institute and State Univ., Blacksburg (USA)
4. Univ. of Southern California, Los Angeles (USA)

Azotobacter vinelandii ferredoxin I is a small protein that contains one (4Fe-4S) cluster and one (3Fe-4S) cluster. Recently the x-ray crystal structure has been redetermined and the fdxA gene, which encodes the protein, has been cloned and sequenced. Here the authors report the site-directed mutation of Cys-20, which is a ligand of the (4Fe-4S) cluster in the native protein, to alanine and the characterization of the protein product by x-ray crystallographic and spectroscopic methods. The data show that the mutant protein again contains one (4Fe-4S) cluster and one (3Fe-4S) cluster. The new (4Fe-4S) cluster obtains its fourth ligand from Cys-24, a free cysteine in the native structure. The formation of this (4Fe-4S) cluster drives rearrangement of the protein structure.

• OSTI ID: 7064361
• Journal Information: Proceedings of the National Academy of Sciences of the United States of America; (USA), Journal Name: Proceedings of the National Academy of Sciences of the United States of America; (USA) Vol. 87:2; ISSN 0027-8424; ISSN PNASA
• Country of Publication: United States
• Language: English

Related Subjects

550201* -- Biochemistry-- Tracer Techniques
59 BASIC BIOLOGICAL SCIENCES
AZOTOBACTER
BACTERIA
COHERENT SCATTERING
CRYSTALLOGRAPHY
DIFFRACTION
DNA
DNA SEQUENCING
FERREDOXIN
METALLOPROTEINS
MICROORGANISMS
MOLECULAR STRUCTURE
MUTAGENESIS
NUCLEIC ACIDS
ORGANIC COMPOUNDS
PROTEINS
RECOMBINANT DNA
SCATTERING
STRUCTURAL CHEMICAL ANALYSIS
X-RAY DIFFRACTION