Chemical and spectroscopic study of ferredoxin l from Azotobacter vinellandii
Thesis/Dissertation
·
OSTI ID:5349389
Ferredoxin l of Azotobacter vinelandii is an iron sulfur protein containing one (4Fe-S) cluster and one (3Fe-3S) cluster. The structure and geometry of the 3Fe cluster is under dispute. The X-ray crystal structure of the protein describes the 3Fe cluster as a planar (3Fe-3S) cluster in which one of the iron atoms is ligated to a solvent accessible oxoligand, presumably from water or hydroxide. Efforts to displace the oxoligand were unsuccessful even when the protein was demonstrably denatured in 80% (V/V) dimethyl sulfoxide. In addition, comparison of the electron spin echo envelopes for H/sub 2/O and D/sub 2/O-equilibrated samples of ferredoxin l showed only a slight deuterium modulation, far less than would be expected were water to be bound as an iron ligand. Studies were also conducted in an effort to characterize the hyperfine-shifted resonances in the proton magnetic resonance spectrum of ferredoxin l. A. vinelandii was grown on synthetic media that contained isotopically labelled cystine as the only sulfur source. In independent experiments ferredoxin l was isolated from organism that was shown on such medium that contained alpha-/sup 2/H-dl-cystine, beta=/sup 2/H-dl-cystine, or betal-/sup 13/C-dl-cystine. However, ferredoxins isolated from these media were devoid of the corresponding isotopic labels. Thus, the experiments designed to characterize the hyperfine-shifted resonances of ferredoxin l were unsuccessful.
- Research Organization:
- City Univ. of New York, NY (USA)
- OSTI ID:
- 5349389
- Country of Publication:
- United States
- Language:
- English
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Related Subjects
550201* -- Biochemistry-- Tracer Techniques
59 BASIC BIOLOGICAL SCIENCES
AMINO ACIDS
AZOTOBACTER
BACTERIA
CARBOXYLIC ACIDS
CHEMICAL SHIFT
CYSTINE
DEUTERIUM
DISULFIDES
HYDROGEN COMPOUNDS
HYDROGEN ISOTOPES
ISOTOPE APPLICATIONS
ISOTOPES
LIGHT NUCLEI
MAGNETIC RESONANCE
MICROORGANISMS
MOLECULAR STRUCTURE
NMR SPECTRA
NUCLEAR MAGNETIC RESONANCE
NUCLEI
ODD-ODD NUCLEI
ORGANIC ACIDS
ORGANIC COMPOUNDS
ORGANIC SULFUR COMPOUNDS
OXYGEN COMPOUNDS
PROTEINS
RESONANCE
SPECTRA
SPIN ECHO
STABLE ISOTOPES
TRACER TECHNIQUES
WATER
59 BASIC BIOLOGICAL SCIENCES
AMINO ACIDS
AZOTOBACTER
BACTERIA
CARBOXYLIC ACIDS
CHEMICAL SHIFT
CYSTINE
DEUTERIUM
DISULFIDES
HYDROGEN COMPOUNDS
HYDROGEN ISOTOPES
ISOTOPE APPLICATIONS
ISOTOPES
LIGHT NUCLEI
MAGNETIC RESONANCE
MICROORGANISMS
MOLECULAR STRUCTURE
NMR SPECTRA
NUCLEAR MAGNETIC RESONANCE
NUCLEI
ODD-ODD NUCLEI
ORGANIC ACIDS
ORGANIC COMPOUNDS
ORGANIC SULFUR COMPOUNDS
OXYGEN COMPOUNDS
PROTEINS
RESONANCE
SPECTRA
SPIN ECHO
STABLE ISOTOPES
TRACER TECHNIQUES
WATER