CD3[sup [minus]] T cells with cis- or trans-acting mutations affecting expression of T cell receptor [beta]-chain mRNA

Journal Article · Tue Dec 15 04:00:00 EST 1992 · Journal of Immunology; (United States)

OSTI ID:7064004

Wong, J G.P.; Nalefski, E A ^[1]; Kasibhatla, S; Rao, A ^[2]

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1. Harvard Medical School, Boston, MA (United States)
2. Dana-Farber Cancer Inst., Boston, MA (United States)

Mutants of an untransformed T cell clone that no longer respond to TCR/CD3 stimulation have been derived using a selection procedure based on the loss of functional response to Ag. This functional selection gives rise to clones of several different phenotypes. The authors have previously described mutants with a TCR/CD3[sup +] cell surface phenotype whose TCR are uncoupled from cellular responses. They describe six additional mutants that do not express TCR/CD3 at the cell surface. One of the CD3[sup [minus]] clones contains a deletion in the successfully rearranged TCR-[alpha] gene, whereas another carries a deletion in the successfully rearranged TCR-[beta] gene. TCR/CD3 expression in these deletion mutants can be restored by transfection of TCR-[alpha] or TCR-[beta] DNA. Four other clones do not express TCR-[beta] mRNA, yet contain no obvious deletions or rearrangements in the TCR-[beta] genes. One of these clones does not transcribe TCR-[beta] chain mRNA. The mutation in this clone does not reside in a trans-acting regulatory element affecting TCR-[beta] gene expression, because the TCR-[beta] mRNA[sup [minus]] phenotype is complemented by fusion with a TCR-[alpha][sup [minus]][beta][sup [minus]] cell line. TCR-[beta] chain regulatory mutants will be valuable in contributing to our understanding of how TCR expression is regulated. 33 refs., 6 figs., 1 tab.

• OSTI ID: 7064004
• Journal Information: Journal of Immunology; (United States), Journal Name: Journal of Immunology; (United States) Vol. 149:12; ISSN JOIMA3; ISSN 0022-1767
• Country of Publication: United States
• Language: English

Related Subjects

550200 -- Biochemistry
550400* -- Genetics
59 BASIC BIOLOGICAL SCIENCES
ANIMAL CELLS
BIOLOGICAL MATERIALS
BLOOD
BLOOD CELLS
BODY FLUIDS
CONNECTIVE TISSUE CELLS
DATA
EXPERIMENTAL DATA
GENE MUTATIONS
GENE REGULATION
INFORMATION
LEUKOCYTES
LYMPHOCYTES
MATERIALS
MEMBRANE PROTEINS
MESSENGER-RNA
MUTATIONS
NUCLEIC ACIDS
NUMERICAL DATA
ORGANIC COMPOUNDS
PROTEINS
RECEPTORS
RNA
SOMATIC CELLS
THYMUS CELLS