The characterization and metabolism of rat hepatic nascent HLD subfractions

Thesis/Dissertation · Fri Jan 01 04:00:00 EST 1988

OSTI ID:7063749

Winkler, K E

Nascent HDL was isolated from recirculating rat liver perfusates and separated by heparin-sepharose chromatography into a non-retained fraction (A) and a fraction (B) that eluted with 0.5 M NaCl. Fractions A and B contained 70% and 30% of the nascent HDL protein, respectively. Livers perfused by the single-pass technique produced fractions A and B in the same ratio as livers perfused by recirculation. The apolipoprotein compositions were similar to those in the recirculating perfusion; however, both fractions A and B had more triglyceride (greater than 50% of total lipid). In a preliminary study designed to investigate whether nascent HDL-apo E was secreted by Kupffer cells or hepatocytes, label was targeted to Kupffer cells by perfusing rat livers with {sup 3}H-acetylated LDL or {sup 3}H-amino acids incorporated into large multilamellar vesicles. For metabolic studies, nascent HDL and nascent VLDL were isolated from rat livers that had been perfused with {sup 3}H-glycerol to label the triglyceride.

• Research Organization: Medical Coll. of Pennsylvania, Philadelphia, PA (USA)
• OSTI ID: 7063749
• Country of Publication: United States
• Language: English

Related Subjects

550501* -- Metabolism-- Tracer Techniques
59 BASIC BIOLOGICAL SCIENCES
ANIMAL CELLS
ANIMALS
BODY
CHROMATOGRAPHY
ESTERS
FRACTIONATION
HYDROGEN COMPOUNDS
ISOTOPE APPLICATIONS
LIPIDS
LIPOPROTEINS
LIVER CELLS
MAMMALS
METABOLISM
ORGANIC COMPOUNDS
ORGANS
PERFUSED ORGANS
PROTEINS
RATS
RODENTS
SEPARATION PROCESSES
SOMATIC CELLS
TRACER TECHNIQUES
TRIGLYCERIDES
TRITIUM COMPOUNDS
VERTEBRATES