Hepatic and renal copper-binding proteins and the identification of proteins affected in the brindled mouse model of Menkes disease
The objective of this thesis were to identify proteins involved in the cellular metabolism of copper, and to identify proteins affected in the brindled mouse model of Menkes disease, and inborn of copper metabolism. Initially, hepatocytes were incubated with {sup 64}Cu(II) and cytosolic {sup 64}Cu-binding proteins were resolved on Sephadex G150 columns. The focus was on cytosolic proteins because this fractions reaches a steady-state before the nuclear and mitochondrial fractions, implying the cytosolic proteins deliver copper to other cell compartments. Four {sup 64}Cu-binding fractions were detected at the earliest times and lowest copper concentrations examined; the void volume fraction, a metallothionein(MT) fraction, and fractions peaking at {approx}88kD(I) and {approx}38kD(II), apparent. Superoxide dismutase eluted after the {approx}38kD {sup 64}Cu-binding fraction. The distributions of {sup 64}Cu and {sup 65}Zn were clearly different. The effects of Cu-status on Cu-binding revealed an inverse relationship between Cu-binding to MT and Cu-binding to proteins in fractions I and II. When MT-levels were low in the cytosols from normal mouse and copper-deficient rat hepatocytes, more Cu-binding was detected in fractions I and II; when MT-levels were high as in neonatal rat hepatocytes and brindled mice kidneys, less Cu-binding was detected in these fractions.
- Research Organization:
- State Univ. of New York, Buffalo, NY (USA)
- OSTI ID:
- 7061604
- Country of Publication:
- United States
- Language:
- English
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Related Subjects
560300* -- Chemicals Metabolism & Toxicology
59 BASIC BIOLOGICAL SCIENCES
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
ANIMAL CELLS
ANIMALS
BETA DECAY RADIOISOTOPES
BETA-MINUS DECAY RADIOISOTOPES
BETA-PLUS DECAY RADIOISOTOPES
BIOSYNTHESIS
CARDIOVASCULAR DISEASES
COPPER
COPPER 64
COPPER ISOTOPES
DAYS LIVING RADIOISOTOPES
DISEASES
ELECTRON CAPTURE RADIOISOTOPES
ELEMENTS
ENZYMES
EVEN-ODD NUCLEI
HEREDITARY DISEASES
HOURS LIVING RADIOISOTOPES
INTERMEDIATE MASS NUCLEI
ISOTOPE APPLICATIONS
ISOTOPES
LIVER CELLS
MAMMALS
METABOLISM
METALLOPROTEINS
METALLOTHIONEIN
METALS
MICE
NERVOUS SYSTEM DISEASES
NUCLEI
ODD-ODD NUCLEI
ORGANIC COMPOUNDS
OXIDOREDUCTASES
PROTEINS
RADIOISOTOPES
RATS
RODENTS
SOMATIC CELLS
SUPEROXIDE DISMUTASE
SYNTHESIS
TRACER TECHNIQUES
TRANSITION ELEMENTS
VERTEBRATES
ZINC 65
ZINC ISOTOPES