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Effects of Cu-metabolic state on the cytosolic distribution of copper in hepatocytes

Conference · · Fed. Proc., Fed. Am. Soc. Exp. Biol.; (United States)
OSTI ID:5125691
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The distribution of /sup 64/Cu among Cu-binding proteins of hepatocytic cytosol was determined after incubating with 2-20 ..mu..M /sup 64/Cu(II) for 1-90 min. Sephadex G150 resolved four /sup 6/2$Cu-containing fractions from rat hepatocytes; Mr greater than or equal to 300 Kd, Mr approx. = 88Kd, Mr approx. = 38Kd and Mr approx. = 10Kd. Higher percentages of /sup 64/Cu were found in the 10Kd and 38Kd fractions at 2 ..mu..M Cu(II) than 10 ..mu..M Cu(II). Mouse hepatocytes showed the same four components. However, relatively less /sup 64/Cu was found in the metallothionein (MT) containing (10Kd) fraction and more in the 88Kd and 33Kd fractions. In the kidney, relatively more /sup 64/Cu was detected in the 88Kd fraction and no /sup 64/Cu was detected in the 38Kd fraction. Cu-deficient rat hepatocytes exhibited a similar distribution as normocupric mouse hepatocytes. Brindled-mouse hepatocytes showed abnormally high Cu in the greater than or equal to 300 Kd and no /sup 64/Cu in the 88Kd fraction. In developing rats (10-20d), most of the cytosolic /sup 64/Cu was in the MT-fraction. Between 20-30Kd, corresponding decreases in MT-/sup 64/Cu and increases in each of the other fractions occurred. The results indicate that one or more components in each of the four fractions is sensitive to copper status. These components may have roles in Cu-metabolism.
Research Organization:
State Univ. of New York, Buffalo
OSTI ID:
5125691
Report Number(s):
CONF-8606151-
Conference Information:
Journal Name: Fed. Proc., Fed. Am. Soc. Exp. Biol.; (United States) Journal Volume: 45:6
Country of Publication:
United States
Language:
English

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Related Subjects

550501* -- Metabolism-- Tracer Techniques
59 BASIC BIOLOGICAL SCIENCES
ANIMAL CELLS
ANIMALS
BETA DECAY RADIOISOTOPES
BETA-MINUS DECAY RADIOISOTOPES
BETA-PLUS DECAY RADIOISOTOPES
COPPER
COPPER 64
COPPER ISOTOPES
DISTRIBUTION
ELECTRON CAPTURE RADIOISOTOPES
ELEMENTS
HOURS LIVING RADIOISOTOPES
INTERMEDIATE MASS NUCLEI
ISOTOPE APPLICATIONS
ISOTOPES
LIVER CELLS
MAMMALS
METABOLISM
METALLOPROTEINS
METALLOTHIONEIN
METALS
MICE
NUCLEI
NUTRITIONAL DEFICIENCY
ODD-ODD NUCLEI
ORGANIC COMPOUNDS
PROTEINS
RADIOISOTOPES
RATS
RODENTS
SOMATIC CELLS
TISSUE DISTRIBUTION
TRACER TECHNIQUES
TRANSITION ELEMENTS
VERTEBRATES