Phorbol ester-inducible T-cell-specific expression of variant mouse mammary tumor virus long terminal repeats

Journal Article · Tue Aug 01 04:00:00 EDT 1989 · Journal of Virology; (USA)

OSTI ID:7059451

Theunissen, H J.M.; Paardekooper, M; Maduro, L J; Michalides, R J.A.M.; Nusse, R ^[1]

---

1. The Netherlands Cancer Institute, Amsterdam (Netherlands)

Acquired proviruses of mouse mammary tumor virus (MMTV) in T-cell leukemias of male GR mice have rearrangements in the U3 region of their long terminal repeats (LTR). In contrast to the endogenous nonrearranged MMTV proviruses, these mutated copies are highly expressed in leukemic T cells. To investigate whether the sequence alterations in the LTR are responsible for the high expression of rearranged MMTV proviruses, the authors made constructs in which normal and variant LTRs drive the bacterial reporter gene chloramphenicol acetyltransferase (CAT). Two different rearranged LTRs were used, one containing a 420-base-pair (bp) deletion (L13) and another carrying a 456-bp deletion plus an 82-bp insertion (L42). These constructs were transfected into murine (GRSL) and human (MOLT-4) T-cell lines that either had or had not been treated with phorbol ester (12-O-tetradecanoylphorbol-13-acetate (TPA)). In GRSL cells, the L13-LTR-CAT construct showed transcriptional activity that was further enhanced by TPA. In MOLT-4 cells, both variant LTRs were active, but only after stimulation with TPA. In contrast, normal(N)-LTR-CAT constructs were not expressed, irrespective of TPA addition. They conclude that the LTR rearrangements generate TPA responsiveness and contribute to T-cell-specific expression of MMTV variants.

• OSTI ID: 7059451
• Journal Information: Journal of Virology; (USA), Journal Name: Journal of Virology; (USA) Vol. 63:8; ISSN 0022-538X; ISSN JOVIA
• Country of Publication: United States
• Language: English

Related Subjects

560300* -- Chemicals Metabolism & Toxicology
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
ANIMAL CELLS
ANIMALS
BIOLOGICAL EFFECTS
BIOLOGICAL MATERIALS
BLOOD
BLOOD CELLS
BODY
BODY FLUIDS
CARCINOGENS
CHEMICAL ACTIVATION
CONNECTIVE TISSUE CELLS
DISEASES
ESTERS
GENE MUTATIONS
GENES
GLANDS
HEMIC DISEASES
IMMUNE SYSTEM DISEASES
LEUKEMIA
LEUKOCYTES
LYMPHOCYTES
MALES
MAMMALS
MAMMARY GLANDS
MAN
MATERIALS
MICE
MICROORGANISMS
MOLECULAR BIOLOGY
MUTATIONS
NEOPLASMS
ONCOGENES
ONCOGENIC VIRUSES
ORGANIC COMPOUNDS
ORGANS
PARASITES
PHORBOL ESTERS
PRIMATES
RODENTS
SOMATIC CELLS
TRANSCRIPTION
VERTEBRATES
VIRUSES