Toxicity of metallic ions and oxides to rabbit alveolar macrophages

Journal Article · Sat Apr 01 04:00:00 EST 1989 · Environmental Research; (USA)

DOI:https://doi.org/10.1016/S0013-9351(89)80039-8· OSTI ID:7059152

Labedzka, M; Gulyas, H; Schmidt, N; Gercken, G ^[1]

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1. Univ. of Hamburg (Germany, F.R.)

The effects of soluble compounds and oxides of As, Cd, Cu, Hg, Ni, Pb, Sb, Sn, V, and Zn on oxidative metabolism and membrane integrity of rabbit alveolar macrophages were studied by 24-hr in vitro exposure. Oxidative metabolism induced by phagocytosis of opsonized zymosan was measured by H{sub 2}O{sub 2} and O{sub 2}{sup {minus}} release and by chemiluminescence in the presence of luminol. Membrane integrity was estimated by extracellular LDH activity. Metallic ions and oxides inhibited the release of active oxygen species. Cd(II), As(III), and V(V) were the most toxic elements as measured by all investigated parameters. Cu(II) decreased O{sub 2}{sup {minus}} release and chemiluminescence effectively but H{sub 2}O{sub 2} release and membrane integrity less. Chemiluminescence was decreased strongly by Hg(II) while O{sub 2}{sup {minus}} and H{sub 2}O{sub 2} release were depressed moderately. Zn(II) and Sb(III) compounds caused medium toxicity and the tested Sn, Ni, and Pb compounds showed only faint toxic effects.

• OSTI ID: 7059152
• Journal Information: Environmental Research; (USA), Journal Name: Environmental Research; (USA) Vol. 48:2; ISSN ENVRA; ISSN 0013-9351
• Country of Publication: United States
• Language: English

Related Subjects

560300* -- Chemicals Metabolism & Toxicology
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
ANIMAL CELLS
ANTIMONY
ARSENIC COMPOUNDS
BODY
CADMIUM COMPOUNDS
CELL CONSTITUENTS
CELL MEMBRANES
CHEMILUMINESCENCE
COMPARATIVE EVALUATIONS
CONNECTIVE TISSUE CELLS
COPPER COMPOUNDS
ELEMENTS
HYDROGEN COMPOUNDS
HYDROGEN PEROXIDE
INJURIES
LEAD COMPOUNDS
LUMINESCENCE
LUNGS
MACROPHAGES
MEMBRANES
MERCURY COMPOUNDS
METABOLISM
METALS
NICKEL COMPOUNDS
NONMETALS
ORGANS
OXYGEN
OXYGEN COMPOUNDS
PEROXIDES
PHAGOCYTES
RADICALS
RESPIRATORY SYSTEM
SOMATIC CELLS
TOXICITY
TRANSITION ELEMENT COMPOUNDS
TRANSITION ELEMENTS
VANADIUM
ZINC