Histamine release due bivalent penicilloyl haptens: control by the number of cross-linked IgE antibodies on the basophil plasma membrane

Journal Article · Sat Jul 01 04:00:00 EDT 1978 · J. Immunol.; (United States)

OSTI ID:7057295

Dembo, M; Goldstein, B; Sobotka, A K; Lichtenstein, L M

We describe the characteristics of in vitro histamine release from human basophils passively sensitized with serum from a penicillin-allergic individual. The histamine release is induced by a synthetic bivalent hapten, bis benzylpenicilloyl 1,6 diaminohexane (BPO)/sub 2/. We present data on the effect of a monovalent hapten, benzylpenicilloyl formyl-L-lysine (BPO)/sub 1/, on the histamine release. We also examine how histamine release depends on the concentration of serum used for passive sensitization, the source of cells used for passive sensitization, and the time allowed for histamine release. We interpret these experiments in terms of a theory of equilibrium binding of bivalent haptens to cell surface antibody that is presented in the previous paper. The results are consistent with the idea that the amount of histamine release is controlled by the number of cross-linked IgE molecules on the cell surface. In particular, the histamine dose-response curve rises because cross-links rise, has a maximum because the cross-links are a maximum, and falls because the cross-links fall.

• Research Organization: Los Alamos Scientific Lab., NM
• OSTI ID: 7057295
• Journal Information: J. Immunol.; (United States), Journal Name: J. Immunol.; (United States) Vol. 121:1; ISSN JOIMA
• Country of Publication: United States
• Language: English

Related Subjects

550200 -- Biochemistry
551000* -- Physiological Systems
59 BASIC BIOLOGICAL SCIENCES
AMINES
ANIMALS
ANTIBIOTICS
ANTIBODIES
ANTIGEN-ANTIBODY REACTIONS
ANTIGENS
AZOLES
BASOPHILS
BINDING ENERGY
BIOLOGICAL MATERIALS
BLOOD
BLOOD CELLS
BODY FLUIDS
CARBOHYDRATES
CELL CONSTITUENTS
CELL MEMBRANES
CHEMICAL REACTIONS
CORRELATIONS
CROSS-LINKING
DRUGS
ENERGY
GLOBULINS
HETEROCYCLIC COMPOUNDS
HISTAMINE
IMIDAZOLES
IMMUNOGLOBULINS
LEUKOCYTES
MAMMALS
MAN
MEMBRANES
ORGANIC COMPOUNDS
ORGANIC NITROGEN COMPOUNDS
PENICILLIN
POLYMERIZATION
POLYSACCHARIDES
PRIMATES
PROTEINS
RECEPTORS
SACCHARIDES
SECRETION
SENSITIVITY
VERTEBRATES